Cell-laden hydrogels in integrated microfluidic devices for long-term cell culture and tubulogenesis assays.
Cell-laden hydrogels in integrated microfluidic devices for long-term cell culture and tubulogenesis assays.
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DOI：
10.1002/smll.201203030
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发表时间：
2013-09
期刊：
影响因子：
13.3
通讯作者：
Nathan P. Gabrielson;A. Desai;B. Mahadik;M. Hofmann;P. Kenis;B. Harley
中科院分区：
文献类型：
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作者：
Nathan P. Gabrielson;A. Desai;B. Mahadik;M. Hofmann;P. Kenis;B. Harley
一种结合了微流控混合和正交补充策略的水凝胶生物芯片被开发和验证，以允许轻松地产生光学透明的3D培养微环境的文库。对胚胎干细胞分化和内皮细胞小管形成的实时芯片跟踪证实，该平台既可用于创建离散3D微环境的社区，也可用于在单细胞和多细胞尺度上对后续的不同反应进行局部监测。
A hydrogel biochip combining microfluidic mixing and orthogonal supplementation strategies is developed and validated to allow facile generation of libraries of optically transparent 3D culture microenvironments. Live, on-chip tracing of embryonic stem cell differentiation and endothelial cell tubulogenesis confirms that the platform can be used to both create communities of discrete 3D microenvironments as well as to locally monitor subsequent divergent responses at both single cell and multi-cell scales.