Co-localization of QTLs for pod fiber content and pod shattering in F2 and backcross populations between yardlong bean and wild cowpea
Co-localization of QTLs for pod fiber content and pod shattering in F2 and backcross populations between yardlong bean and wild cowpea
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DOI：
10.1007/s11032-016-0505-8
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发表时间：
2016-06
影响因子：
3.1
通讯作者：
Wipawan Suanum;P. Somta;Alisa Kongjaimun;Tarika Yimram;A. Kaga;N. Tomooka;Yu Takahashi;P. Srinives
中科院分区：
文献类型：
--
作者：
Wipawan Suanum;P. Somta;Alisa Kongjaimun;Tarika Yimram;A. Kaga;N. Tomooka;Yu Takahashi;P. Srinives
长豇豆[Vigna unguiculata（L.）哇长爪亚种gr.sesquipedalis]是一种蔬菜豆类作物，由栽培谷物豇豆（V. unguiculatassp.unguiculatacv.-）进化而来gr.unguiculata），其从野生豇豆驯化而来。与其野生祖先相比，它在豆荚长度和豆荚纤维含量上发生了巨大的变化，并且完全丧失了豆荚破碎。本研究以长豇豆与野生豇豆杂交的两个群体（BC 1F 1和F2）为材料，对控制角果纤维含量和落荚率的QTL进行了定位。在田间条件下，测定了BC 1F 1：2和F2：3家系成熟荚果中不溶性膳食纤维（纤维素、半纤维素和木质素）含量和荚果破碎率。相关分析表明，纤维类型之间以及纤维与落粒率之间均呈正相关。包含性复合区间作图结果表明，位于连锁群7（LG 7）上的主效QTL控制荚果纤维素、半纤维素和木质素含量以及荚果落粒率。LG 1和LG 4上的其他与荚纤维相关的QTL也与落荚相关的QTL共定位。与小豆（Vignaangularis）基因组比较分析表明，LG 7上的纤维素、半纤维素、木质素和荚果脱落QTL区域含有编码MYB转录因子MYB 83的基因，而LG 1上的纤维素和荚果脱落QTL区域含有编码纤维素合成酶A7（CESA 7）的基因。这些基因可能是功能研究的重要目标，以揭示影响角果纤维生物合成和角果脱落的主要因素。
Yardlong bean [Vigna unguiculata(L.) Walp. ssp.unguiculatacv.-gr.sesquipedalis] is a vegetable legume crop evolved from cultivated grain cowpea (V. unguiculatassp.unguiculatacv.-gr.unguiculata) which is domesticated from wild cowpea. It has a dramatic change in pod length and pod fiber content, and a complete loss of pod shattering, as compared to its wild progenitor. In this study, we identified quantitative trait loci (QTLs) controlling pod fiber content and pod shattering in two populations (BC1F1and F2) derived from a cross between yardlong bean and wild cowpea. BC1F1:2and F2:3families were grown under field condition in which insoluble dietary fiber (cellulose, hemicelluloses and lignin) contents in mature pods, and pod shattering were evaluated. Correlation analysis showed positive relationship among the types of fiber, and between the fiber and shattering. Inclusive composite interval mapping revealed that a major QTL on linkage group 7 (LG7) controlled cellulose, hemicellulose and lignin contents in pod, and pod shattering. The other QTLs related to pod fibers on LG1 and LG4 also co-localized with the QTLs for pod shattering. Comparative genome analysis with azuki bean (Vigna angularis) suggested that the QTL region on LG7 for cellulose, hemiclellulose, lignin and pod shattering in yardlong bean contains genes encoding MYB transcription factor, MYB83, regulating biosynthesis of the three fibers, while the QTL region for cellulose and shattering of pod on LG1 harbors gene encoding cellulose synthase A7 (CESA7). These genes may be important targets for functional study to reveal major factors regulating pod fiber biosynthesis and pod shattering in yardlong bean.