The HIV-1 long terminal repeat contains an unusual element that induces the synthesis of short RNAs from various mRNA and snRNA promoters.
The HIV-1 long terminal repeat contains an unusual element that induces the synthesis of short RNAs from various mRNA and snRNA promoters.
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DOI：
10.1101/gad.4.12a.2061
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发表时间：
1990-12
影响因子：
10.5
通讯作者：
R. Ratnasabapathy;Michael Sheldon;Lynn Johal;Nouria Hernandez
中科院分区：
文献类型：
--
作者：
R. Ratnasabapathy;Michael Sheldon;Lynn Johal;Nouria Hernandez
We describe an unusual element that activates the synthesis of short transcripts from a wide variety of mRNA and small nuclear RNA (snRNA) promoters, including the U6 RNA polymerase III promoter. This inducer of short transcripts (IST) is located between positions -5 and +82 relative to the cap site in the HIV-1 LTR. In the presence of IST, the total transcriptional activity of the different promoters is greatly increased, but the resulting additional RNA molecules are short, ending around position +60. IST is not the RNA target (TAR) for Tat trans-activation; however, because it relies entirely on cellular factors for activity, IST may serve to provide abundant RNA targets for Tat trans-activation without a requirement for full-length viral mRNA expression.
