Measuring gluconeogenesis using a low dose of 2H2O:: advantage of isotope fractionation during gas chromatography
Measuring gluconeogenesis using a low dose of 2H2O:: advantage of isotope fractionation during gas chromatography
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DOI：
10.1152/ajpendo.00485.2002
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发表时间：
2003-05-01
影响因子：
5.1
通讯作者：
Previs, SF
中科院分区：
文献类型：
--
作者：
Katanik, J;McCabe, BJ;Previs, SF
通过用（H2O）-H-2使体内水富集至接近0.5%H-2，并测定与血糖的碳-5结合的H-2与碳-2的比率，可以测量葡萄糖生成对葡萄糖产生的贡献。在相应的葡萄糖碳转化为甲醛然后转化为六亚甲基四胺（HMT）之后，可以使用气相色谱-质谱法测量该标记比率。我们提出了一种用于整合离子色谱图的技术，该技术允许仅使用体液中的0.05% H-2（即，比当前剂量低10倍）。该技术利用天然标记HMT和[H-2] HMT的气相色谱保留时间差异。我们讨论了使用低剂量的（H2O）-H-2的优点（S），以量化的贡献的异生。
The contribution of gluconeogenesis to glucose production can be measured by enriching body water with (H2O)-H-2 to similar to0.5% H-2 and determining the ratio of H-2 that is bound to carbon-5 vs. carbon-2 of blood glucose. This labeling ratio can be measured using gas chromatography-mass spectrometry after the corresponding glucose carbons are converted to formaldehyde and then to hexamethylenetetramine (HMT). We present a technique for integrating ion chromatograms that allows one to use only 0.05% H-2 in body water (i.e., 10 times less than the current dose). This technique takes advantage of the difference in gas chromatographic retention times of naturally labeled HMT and [H-2] HMT. We discuss the advantage(s) of using a low dose of (H2O)-H-2 to quantify the contribution of gluconeogenesis.