Insertion of a CACTA-like transposable element disrupts the function of the BoCCD4 gene in yellow-petal Chinese kale
Insertion of a CACTA-like transposable element disrupts the function of the BoCCD4 gene in yellow-petal Chinese kale
复制标题
类似 CACTA 转座元件的插入破坏了黄瓣羽衣甘蓝 BoCCD4 基因的功能
DOI：
10.1007/s11032-019-1008-1
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发表时间：
2019-09-01
影响因子：
3.1
通讯作者：
Zhang, Yangyong
中科院分区：
文献类型：
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作者：
Zhang, Bin;Han, Fengqing;Zhang, Yangyong
甘蓝包含几个重要的亚种，包括卷心菜、西兰花、花椰菜、羽衣甘蓝和大头菜。羽衣甘蓝的花瓣颜色多为白色，有时呈黄色。为了探索羽衣甘蓝花瓣颜色变异的遗传基础，通过两个自交系2114（黄色花瓣）和2116（白色花瓣）的杂交构建了F-2和BC1（回交）群体。 F-2 和 BC1 群体的遗传分析表明，黄色花瓣颜色由单个隐性核基因（称为 cpc-2）控制。根据亲本重测序数据设计的插入删除 (InDel) 标记用于定位 cpc-2。精细定位结果表明，cpc-2基因位于C03染色体上569 kb区间，两侧为InDel标记ZB636和ZB692，遗传距离分别为0.3 cM和0.6 cM。通过分析该区间基因的核苷酸变异和注释，预测CCD4家族基因是cpc-2的候选基因，并更名为BoCCD4.2。此外，CACTA样转座元件（TE3）的插入中断了BoCCD4基因的功能，这可能导致BoCCD4功能丧失以及花瓣颜色从白色转变为黄色。 BoCCD4基因中的TE3插入也存在于159个材料中的63个甘蓝自交系中，这表明BoCCD4的TE3型无效等位基因在甘蓝和羽衣甘蓝两个亚种分化之前形成，并且羽衣甘蓝的进化比甘蓝早得多。该研究为克隆BoCCD4.2和揭示羽衣甘蓝花瓣颜色形成的分子机制奠定了基础。
Brassica oleracea comprises several important subspecies, including cabbage, broccoli, cauliflower, Chinese kale, and kohlrabi. The petal color of Chinese kale is mostly white and sometimes yellow. To explore the genetic basis of petal color variation in Chinese kale, F-2 and BC1 (backcross) populations were constructed from the cross of two inbred lines, 2114 (yellow petal) and 2116 (white petal). Genetic analysis of the F-2 and BC1 populations demonstrated that yellow petal color was controlled by a single recessive nuclear gene, termed cpc-2. Insertion-deletion (InDel) markers, designed based on the parental resequencing data, were used to map cpc-2. The fine mapping results indicated that the cpc-2 gene was located in a 569-kb interval on chromosome C03 flanked by InDel markers ZB636 and ZB692, with genetic distances of 0.3 cM and 0.6 cM, respectively. By analyzing the nucleotide variations and annotations of the genes in this interval, a CCD4 family gene was predicted to be a candidate for cpc-2 and renamed BoCCD4.2. In addition, insertion of the CACTA-like transposable element (TE3) interrupted the function of the BoCCD4 gene, which may have resulted in the loss of function of BoCCD4 and the petal color transition from white to yellow. The TE3 insertion in the BoCCD4 gene was also present in 63 cabbage inbred lines among 159 accessions, which revealed that the TE3-type null allele of BoCCD4 formed before the divergence of the two subspecies cabbage and Chinese kale and that Chinese kale evolved much earlier than cabbage. This study lays the foundation for cloning BoCCD4.2 and revealing the molecular mechanism underlying petal color formation in Chinese kale.