TaqMan low density array is roughly right for gene expression quantification in colorectal cancer
TaqMan low density array is roughly right for gene expression quantification in colorectal cancer
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DOI：
10.1016/j.cca.2007.12.009
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发表时间：
2008-03-01
影响因子：
5
通讯作者：
Lai, Maode
中科院分区：
文献类型：
--
作者：
Lue, Bingjian;Xu, Jing;Lai, Maode
背景资料：TaqMan低密度芯片（LDA）可同时检测多个基因的mRNA表达，有望用于功能基因组学的高通量筛选。方法：应用LDA技术检测27例结直肠癌组织中95个胃肠分化相关基因的mRNA表达。结果：LDA扩增成功率为97.2%（11,520/11,844），与常规Q-PCR方法相比，差异表达基因的检出率明显降低（P <0.05）。在所有基因样本中，LDA内检测之间完全一致（CCC=0.952，p 0.713）。低Ct值基因（30个循环）（p
Background: TaqMan low density array (LDA) is promising for high throughput screening in functional genomics by simultaneously measuring mRNA expression of multiple genes. However, the reproducibility and reliability remain to be explored.Methods: We applied LDA to detect mRNA expression of 95 gastrointestinal differentiation associated genes in 27 colorectal cancers with individual-matched normal mucosa. Conventional Q-PCR assay was done to detect 18 differentially expressed genes in additional 22 colorectal cancers.Results: A total of 97.2% (11,520/11,844) gene samples were successfully amplified by LDA. There was a perfect agreement between intra-LDA assays in all gene samples (CCC=0.952, p0.713). Genes with low Ct values (30 cycles) (p