TOWARDS CONSTRUCTION OF A HIGH-RESOLUTION MAP OF THE MOUSE GENOME USING PCR-ANALYZED MICROSATELLITES
TOWARDS CONSTRUCTION OF A HIGH-RESOLUTION MAP OF THE MOUSE GENOME USING PCR-ANALYZED MICROSATELLITES
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DOI：
10.1093/nar/18.14.4123
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发表时间：
1990-07-25
影响因子：
14.9
通讯作者：
TODD, JA
中科院分区：
文献类型：
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作者：
LOVE, JM;KNIGHT, AM;TODD, JA
用聚合酶链反应和凝胶电泳分析了小鼠基因组数据库中含有简单重复序列或微卫星的50个序列的大小变异。88%的序列，其中大部分含有二核苷酸重复，CA/GT，显示大小之间的不同近交系小鼠和野生小鼠，小鼠spretus的变化。62%的序列有3个或3个以上等位基因。含GA/CT和AT/TA的序列也是可变的。这些大小变异体中约有一半可通过琼脂糖凝胶电泳检测到。这种简单的方法在连锁和基因组作图研究中非常有用，并将有助于构建小鼠和人类基因组的高分辨率图谱。
Fifty sequences from the mouse genome database containing simple sequence repeats or microsatellites have been analysed for size variation using the polymerase chain reaction and gel electrophoresis. 88% of the sequences, most of which contain the dinucleotide repeat, CA/GT, showed size variations between different inbred strains of mice and the wild mouse, Mus spretus. 62% of sequences had 3 or more alleles. GA/CT and AT/TA-containing sequences were also variable. About half of these size variants were detectable by agarose gel electrophoresis. This simple approach is extremely useful in linkage and genome mapping studies and will facilitate construction of high resolution maps of both the mouse and human genomes.