Single marker and haplotype analysis of the chicken apolipoprotein B gene T123G and D9500D9- polymorphism reveals association with body growth and obesity.
Single marker and haplotype analysis of the chicken apolipoprotein B gene T123G and D9500D9- polymorphism reveals association with body growth and obesity.
复制标题
DOI：
10.1093/ps/85.2.178
复制
发表时间：
2006-02
期刊：
影响因子：
4.4
通讯作者：
S. Zhang;Hui Li;H. Shi
中科院分区：
文献类型：
--
作者：
S. Zhang;Hui Li;H. Shi
载脂蛋白 B (apoB) 在哺乳动物小肠和肝脏中合成，在富含甘油三酯的脂蛋白的组装和分泌中发挥重要作用，是极低密度脂蛋白、中密度脂蛋白和低密度脂蛋白 (LDL) 的组成部分，也是 LDL 受体的配体。本研究旨在探讨apoB基因对鸡生长和脂肪组织沉积的影响。使用腹部脂肪不同的东北农业大学肉鸡品系。发现了两个新的多态性，即第26外显子123处的同义突变T-->G和apoB基因终止码TGA后500 bp处的9 bp缺失，然后通过单标记和单倍型分析检测这2个多态性与性状之间的关联。结果表明，apoB基因的2个多态性的单倍型与影响身体生长和肥胖性状的潜在主要位点或基因连锁。本研究的结果不仅表明apoB基因在鸡中具有主要功能，而且还表明与apoB基因相关的分子遗传标记可用于低腹部脂肪的选择程序。
Apolipoprotein B (apoB) is synthesized in the mammalian small intestine and liver, where it serves an essential role in the assembly and secretion of triglyceride-rich lipoproteins and is the constituent of very low density lipoprotein, intermediate density lipoprotein, and low density lipoprotein (LDL), and is the ligand for the LDL receptor. The present study was designed to investigate the effects of the apoB gene on chicken growth and deposition of adipose tissues. The Northeast Agricultural University broiler lines divergently selected for abdominal fat were used. Two novel polymorphisms, a synonymous mutation T-->G at 123 of 26th exon, and a 9 bp deletion at 500 bp after the stop code TGA of the apoB gene were found, and then the association between these 2 polymorphisms and traits were detected using both single marker and haplotype analysis. Results showed that the haplotype of the 2 polymorphisms of apoB gene was linked with potential major loci or genes affecting the body growth and fatness traits. The results of the present study not only suggest a primary function of apoB gene in the chicken, but also suggest that the use of molecular genetic markers associated with the apoB gene can be used in a selection program for low abdominal fat.