Fungal symbiosis in rice requires an ortholog of a legume common symbiosis gene encoding a Ca2+/calmodulin-dependent protein kinase1[OA]
Fungal symbiosis in rice requires an ortholog of a legume common symbiosis gene encoding a Ca2+/calmodulin-dependent protein kinase1[OA]
复制标题
DOI：
10.1104/pp.107.109876
复制
发表时间：
2007-12-01
期刊：
影响因子：
7.4
通讯作者：
Zhu, Hongyan
中科院分区：
文献类型：
--
作者：
Chen, Caiyan;Gao, Muqiang;Zhu, Hongyan
在自然生态系统中，许多植物能够与微生物建立互利共生关系。对可持续农业至关重要的是超过 80% 的陆地植物与丛枝菌根 (AM) 真菌之间以及豆科植物与固氮根瘤菌之间形成的共生关系。有趣的是，这两种共生体在豆科植物中共享重叠的信号通路，这表明进化上最近的根瘤共生体可能从古代 AM 共生体中获得了功能。豆科植物中的蒺藜苜蓿 DMI3（不引起感染）基因 (MtDMI3) 及其直系同源物是细菌和真菌共生所必需的。 MtDMI3 编码 Ca2+/钙调蛋白依赖性蛋白激酶 (CCaMK)，对于 Nod 因子感知诱导的 Ca2+ 信号转导至关重要。 MtDMI3 的推定直系同源物也存在于非豆科植物中，但它们在 AM 共生中的功能尚未在任何非豆科物种中得到证实。在这里，我们结合反向遗传方法和跨物种互补测试来表征 MtDMI3 的水稻 (Oryza sativa) 直向同源物（即 OsDMI3）在 AM 共生中的功能。我们证明 OsDMI3 不仅是水稻 AM 共生所必需的，而且能够补充蒺藜麦 dmi3 突变体，表明 MtDMI3 直系同源物在非豆科植物中具有同等作用。
In natural ecosystems, many plants are able to establish mutually beneficial symbioses with microorganisms. Of critical importance to sustainable agriculture are the symbioses formed between more than 80% of terrestrial plants and arbuscular mycorrhizal (AM) fungi and between legumes and nitrogen-fixing rhizobial bacteria. Interestingly, the two symbioses share overlapping signaling pathways in legumes, suggesting that the evolutionarily recent root nodule symbiosis may have acquired functions from the ancient AM symbiosis. The Medicago truncatula DMI3 (DOESN'T MAKE INFECTIONS3) gene (MtDMI3) and its orthologs in legumes are required for both bacterial and fungal symbioses. MtDMI3 encodes a Ca2+/calmodulin-dependent protein kinase (CCaMK) essential for the transduction of the Ca2+ signal induced by the perception of Nod factors. Putative orthologs of MtDMI3 are also present in non-legumes, but their function in AM symbiosis has not been demonstrated in any non-legume species. Here, we combine reverse genetic approaches and a cross-species complementation test to characterize the function of the rice (Oryza sativa) ortholog of MtDMI3, namely, OsDMI3, in AM symbiosis. We demonstrate that OsDMI3 is not only required for AM symbiosis in rice but also is able to complement a M. truncatula dmi3 mutant, indicating an equivalent role of MtDMI3 orthologs in non-legumes.