Expression analysis of G Protein-Coupled Receptors in mouse macrophages.
Expression analysis of G Protein-Coupled Receptors in mouse macrophages.
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DOI：
10.1186/1745-7580-4-5
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发表时间：
2008-04-29
期刊：
影响因子：
--
通讯作者：
Sweet MJ
中科院分区：
文献类型：
--
作者：
Lattin JE;Schroder K;Su AI;Walker JR;Zhang J;Wiltshire T;Saijo K;Glass CK;Hume DA;Kellie S;Sweet MJ
单核细胞和巨噬细胞表达广泛的G蛋白偶联受体(GPCRs)，调节炎症和免疫。在这项研究中，我们对原代小鼠巨噬细胞中GPCR的表达进行了系统的微阵列分析，以确定与一组其他类型的细胞相比，哪些家族成员富含巨噬细胞，或者受到炎性刺激--细菌产物脂多糖(LPS)的调节。P2RY家族的几个成员在巨噬细胞中有显著的表达模式；P2ry6mRNA基本上是以巨噬细胞特有的方式表达，而P2ry1和P2ry5mRNA的表达水平被脂多糖强烈下调。其他几个GPCRs的表达或者仅限于巨噬细胞(例如Gpr84)，或者同时在巨噬细胞和神经组织中表达(例如P2ry12，GPR85)。骨髓来源的巨噬细胞和硫代乙醇酸诱导的腹膜巨噬细胞表达的GPCR谱具有一定的共性，但也有几个GPCRs在这两个细胞群中优先表达。在这项研究中发现的几种GPCRs在巨噬细胞中的组成性或受调控的表达以前没有被描述过。未来对这种GPCRs及其激动剂的研究可能会为巨噬细胞生物学提供重要的见解，以及可能成为未来药物发现目标的新的炎症途径。
Monocytes and macrophages express an extensive repertoire of G Protein-Coupled Receptors (GPCRs) that regulate inflammation and immunity. In this study we performed a systematic micro-array analysis of GPCR expression in primary mouse macrophages to identify family members that are either enriched in macrophages compared to a panel of other cell types, or are regulated by an inflammatory stimulus, the bacterial product lipopolysaccharide (LPS). Several members of the P2RY family had striking expression patterns in macrophages; P2ry6 mRNA was essentially expressed in a macrophage-specific fashion, whilst P2ry1 and P2ry5 mRNA levels were strongly down-regulated by LPS. Expression of several other GPCRs was either restricted to macrophages (e.g. Gpr84) or to both macrophages and neural tissues (e.g. P2ry12, Gpr85). The GPCR repertoire expressed by bone marrow-derived macrophages and thioglycollate-elicited peritoneal macrophages had some commonality, but there were also several GPCRs preferentially expressed by either cell population. The constitutive or regulated expression in macrophages of several GPCRs identified in this study has not previously been described. Future studies on such GPCRs and their agonists are likely to provide important insights into macrophage biology, as well as novel inflammatory pathways that could be future targets for drug discovery.