Muscarinic receptor binding in the mouse heart: the selective modulatory effect of fluoride ion on agonist binding.
Muscarinic receptor binding in the mouse heart: the selective modulatory effect of fluoride ion on agonist binding.
复制标题
小鼠心脏中毒蕈碱受体的结合：氟离子对激动剂结合的选择性调节作用。
DOI：
10.1016/0024-3205(82)90602-6
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发表时间：
1982
期刊：
影响因子：
6.1
通讯作者：
Roeske,WR
中科院分区：
文献类型：
--
作者：
Barritt,D;Yamamura,HI;Roeske,WR
研究了氟离子对特异性毒蕈碱激动剂配体[3H]c甲基二氧戊环([3H]CD)与小鼠心脏毒蕈碱受体结合的影响。利用平衡配体结合实验，氟化钠 (10mM) 可将 [3H]CD 结合降低 52%（浓度为 2 nM 时测量）。对几种不同离子的研究表明，[3H]CD 结合的减少是氟化物的特定作用。这种氟化物调节对于激动剂结合具有选择性，因为没有观察到氟化物对毒蕈碱拮抗剂[3H](-)奎宁环基苯甲酸(QNB)的结合的影响。
The effect of fluoride ion on the binding of the specific muscarinic agonist ligand [3H]c is methyldioxolane ([3H]CD) to the mouse cardiac muscarinic receptor was investigated. Utilizing equilibrium ligand binding experiments, sodium fluoride (10mM) was shown to decrease [3H]CD binding, measured at a concentration of 2 nM, by 52%. Studies with several different ions demonstrated that the reduction in [3H]CD binding was a specific effect of fluoride. This fluoride modulation was selective for agonist binding, as no effect of fluoride on the binding of the muscarinic antagonist [3H](−) quinuclidinyl benzilate (QNB) was observed.