Pre- and postnatal hepatic gene expression profiles of two pig breeds differing in body composition: insight into pathways of metabolic regulation
Pre- and postnatal hepatic gene expression profiles of two pig breeds differing in body composition: insight into pathways of metabolic regulation
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DOI：
10.1152/physiolgenomics.00178.2006
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发表时间：
2007-05-11
影响因子：
4.6
通讯作者：
Wimmers, Klaus
中科院分区：
文献类型：
--
作者：
Ponsuksili, Siriluck;Murani, Eduard;Wimmers, Klaus
肝脏在调节代谢状态、营养物质分配和能量消耗方面起着核心作用。为了深入了解肝脏代谢途径和影响体组成相关性状的关键转录本，研究人员比较了肥胖的德国长白猪（DL）和瘦弱的彼得兰猪（Pi）两个品种的肝脏表达谱。将猪寡核苷酸芯片与发育期（180日龄）和产前（受孕后35、63和91天）获得的肝脏crna进行杂交，这些阶段代表肝脏的三个发育阶段，即分化期、代谢活动期和糖原蓄积期。在DL和Pi之间调节的基因数量方面，最显著的差异是在青春期前后，肥胖DL猪的脂质代谢途径关键基因（FASN、ACSS2、ACACA）上调，而瘦弱Pi猪的细胞生长和/或维持、蛋白质合成以及细胞增殖途径基因（PPARD、POU1F1、IGF2R）上调。此外，品种依赖性表达谱的时间过程分析显示，从产前阶段到青春期周围，涉及脂质途径和细胞活性的生物过程的基因具有品种典型的时间调节，即品种差异已经在产前早期发育中开始。关于两个品种在身体组成、营养和能量分配和利用方面的差异的mRNA表达水平的信息揭示了与肥胖和瘦相关性状的功能候选基因。
The liver plays a central role in the regulation of the metabolic status, partitioning of nutrients, and expenditure of energy. To gain insight into hepatic metabolic pathways and key transcripts affecting traits related to body composition, liver expression profiles were compared of pigs of two breeds, the obese German Landrace (DL) and the lean Pietrain (Pi). Porcine oligonucleotide microarray were hybridized with liver cRNAs obtained at peripubertal age (180 days of age) and prenatal stages (35, 63, and 91 days postconception) that represent three developmental stages of liver, i.e., period of differentiation, period of metabolic activity, and period of glycogen accumulation. In terms of the number of genes regulated between DL and Pi, the most striking distinctions were found at peripubertal age with upregulation of key genes of lipid metabolism pathways (FASN, ACSS2, ACACA) in obese DL pigs and upregulation of genes of cell growth and/or maintenance, and protein syntheses, as well as cell proliferation pathways (PPARD, POU1F1, IGF2R), in lean Pi pigs. Moreover, time course analysis of breed-dependent expression profiles revealed breed-typical temporal regulation from prenatal stages to peripubertal age of genes assigned to biological processes involving lipid pathways and cell activity, i.e., breed differences are already initiated during early prenatal development. Information about mRNA expression levels of the two breeds differing in body composition, partitioning and utilization of nutrients and energy reveals functional candidate genes for traits related to obesity and leanness.