Skewed immunological balance between Th17 (CD4+IL17A+) and Treg (CD4+CD25+FOXP3+) cells in human oral squamous cell carcinoma
Skewed immunological balance between Th17 (CD4+IL17A+) and Treg (CD4+CD25+FOXP3+) cells in human oral squamous cell carcinoma
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DOI：
10.1007/s13402-012-0093-5
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发表时间：
2012-10-01
影响因子：
6.6
通讯作者：
Das, Satya Narayan
中科院分区：
文献类型：
--
作者：
Gaur, Poonam;Qadir, Gulam Abdul;Das, Satya Narayan
一些研究记录了Th17和T调节(Treg)细胞在各种人类恶性肿瘤中的调节，这种调节可能会随着疾病的类型和程度而变化。然而，口腔癌患者的这类数据很少，因此本研究旨在阐述口腔癌患者这两个T细胞亚群之间的免疫平衡。我们分析了45例口腔鳞癌患者和40名健康志愿者外周血中的各种T细胞亚群。我们发现，与健康对照组相比，患者Th17(+)IL 17A(+)和Treg(CD 4(+)CD25(+)FOXP3(+)细胞的比例均显著升高(p<；0.0001)，这进一步显示了患者与临床病理参数之间的相互平衡。我们还在患者和健康对照组中检测到这些细胞的循环CD8(+)亚群，尽管两组之间的差异在统计学上并不显著。Th17细胞在早期和无淋巴结转移的患者中发生率较高，而Tregs的发生率与临床分期和淋巴结转移有关。Th17细胞与CD4(+)T细胞、CD8(+)T细胞数量呈正相关，与Tregs呈负相关。相反，Tregs与CD4(+)T细胞和CD8(+)T细胞呈负相关。我们的结果表明，在口腔癌早期，Th17/Tregs比率升高，而在高阶段口腔癌中这一比率下降。Th17和Tregs的这种反向调节可能是口腔癌患者的一个重要预后因素。
Several studies have documented modulation of Th17 and T regulatory (Treg) cells in various human malignancies which may vary with the type and extent of the disease. However, such data in patients with oral cancer is scarce and hence the current study was designed to elaborate the immunological balance between these two T cell subsets in oral cancer.We analyzed various T cell subsets in the peripheral blood of 45 oral squamous cell carcinoma (OSCC) patients and 40 healthy volunteers. We found that, compared with the healthy controls, patients had a significantly (p < 0.0001) higher proportion of both Th17 (CD4(+)IL17A(+)) and Treg (CD4(+)CD25(+)FOXP3(+)) cells, which further showed a reciprocal balance in relation to clinico-pathological parameters in patients. We also detected a circulating CD8(+) subset of these cells in both patients and healthy controls, although the difference between the two groups was statistically insignificant. Higher frequencies of Th17 cells were found in patients with early stages and without lymph node involvement, while an increased prevalence of Tregs was associated with higher clinical stages and lymph node involvement. Moreover, Th17 cells were quantitatively and positively correlated to CD4(+)T and CD8(+)T cells and inversely correlated with Tregs. Contrarily, Tregs showed a negative association with CD4(+)T and CD8(+)T cells.Our results suggest an increase in Th17/Tregs ratio in early stages and a decrease in this ratio in higher stages of oral cancer. Such counter regulation of Th17 and Tregs may be a significant prognostic factor in oral cancer patients.