The SQV-1 UDP-glucuronic acid decarboxylase and the SQV-7 nucleotide-sugar transporter may act in the Golgi apparatus to affect Caenorhabditis elegans vulval morphogenesis and embryonic development
The SQV-1 UDP-glucuronic acid decarboxylase and the SQV-7 nucleotide-sugar transporter may act in the Golgi apparatus to affect Caenorhabditis elegans vulval morphogenesis and embryonic development
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DOI：
10.1073/pnas.172522199
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发表时间：
2002-10-29
影响因子：
11.1
通讯作者：
Horvitz, HR
中科院分区：
文献类型：
--
作者：
Hwang, HY;Horvitz, HR
近年来的研究表明，糖胺聚糖在动物的发育过程中起着重要的作用。基因sqv-3、sqv-7和sqv-8是秀丽隐杆线虫外阴形态发生所必需的，影响软骨素和硫酸乙酰肝素糖胺聚糖的生物合成。我们克隆了SQV-1，并表明SQV-1蛋白是一种II型跨膜蛋白，其功能是UDP-葡萄糖醛酸脱羧酶。SQV-1定位于点状细胞质区室，并与SQV-7核糖转运蛋白共定位，其可能作用于高尔基体。SQV-1和SQV-7都在外阴和卵母细胞中表达，它们可能分别在外阴形态发生和胚胎发育中起作用。SQV-7和SQV-1无效突变体的后代未能启动胞质分裂，可能是因为它们无法将质膜与蛋壳分离，这是一种类似于外阴不完全内陷的缺陷。
Recent findings indicate that glycosaminoglycans can play important roles in animal development. The genes sqv-3,-7, and -8, which are necessary for vulval morphogenesis in Caenorhabditis elegans, affect the biosynthesis of chondroitin and heparan sulfate glycosaminoglycans. We cloned sqv-1 and showed that the SQV-1 protein is a type II transmembrane protein that functions as a UDP-glucuronic acid decarboxylase. SQV-1 localizes to punctate cytoplasmic compartments and colocalizes with the SQV-7 nucleoticle-sugar transporter, which probably acts in the Golgi apparatus. SQV-1 and SQV-7 are both expressed in the vulva and in oocytes, where they likely act in vulval morphogenesis and embryonic development, respectively. Progeny of sqv-7 and sqv-1 null mutants fail to initiate cytokinesis, possibly because they are unable to separate the plasma membrane from the eggshell, a defect analogous to that of incomplete vulval invagination.