Long noncoding RNA and phase separation in cellular stress response
Long noncoding RNA and phase separation in cellular stress response
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细胞应激反应中的长非编码RNA和相分离
DOI：
10.1093/jb/mvab156
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发表时间：
2022
期刊：
影响因子：
--
通讯作者：
Akimitsu Nobuyoshi
中科院分区：
文献类型：
--
作者：
Onoguchi-Mizutani Rena;Akimitsu Nobuyoshi
应激反应对于感知和适应环境变化非常重要。近年来，通过液-液分离形成的一种无膜细胞器--RNA-蛋白质(RNP)凝聚体被提出用来调节应激反应。由于RNP缩合物是通过带正电荷的蛋白质和带负电荷的RNA之间的相互作用形成的，因此蛋白质和RNA的比例对于相分离的缩合物的形成至关重要。特别是，由于其二级结构和较长的长度，长非编码RNA(LncRNAs)可以有效地成核相分离的RNP凝聚体。因此，由于lncRNAs在胁迫响应下通过相分离作为生物凝聚体的潜在调节因子而受到越来越多的关注。本文对lncRNAs参与应激反应下RNP凝聚体形成的研究现状进行了综述。我们还证明了lncRNA驱动的相分离为理解对几种细胞压力的反应提供了有用的基础。
Stress response is important for sensing and adapting to environmental changes. Recently, RNA-protein (RNP) condensates, which are a type of membrane-less organelle formed by liquid–liquid phase separation, have been proposed to regulate the stress response. Because RNP condensates are formed through interactions between positively charged proteins and negatively charged RNAs, the ratio of proteins to RNAs is critical for phase-separated condensate formation. In particular, long noncoding RNAs (lncRNAs) can efficiently nucleate phase-separated RNP condensates because of their secondary structure and long length. Therefore, increased attention has been paid to lncRNAs because of their potential role as a regulator of biological condensates by phase separation under stress response. In this review, we summarize the current research on the involvement of lncRNAs in the formation of RNP condensates under stress response. We also demonstrate that lncRNA-driven phase separation provides a useful basis to understanding the response to several kinds of cellular stresses.