Polyamines Protect Rat Embryo in vitro from High Glucose-induced Developmental Delay and Dysmorphogenesis
Polyamines Protect Rat Embryo in vitro from High Glucose-induced Developmental Delay and Dysmorphogenesis
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DOI：
10.1002/bdrb.20182
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发表时间：
2009-02-01
影响因子：
--
通讯作者：
Palomar-Morales, Martin
中科院分区：
文献类型：
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作者：
Chirino-Galindo, Gladys;Baiza-Gutman, Luis A.;Palomar-Morales, Martin
背景：患有糖尿病的哺乳动物怀孕会导致低出生体重、畸形和宫内死亡。胃肠外应用天然多胺或其前体，L-精氨酸，糖尿病妊娠大鼠，部分防止糖尿病引起的发育改变。本实验旨在了解这种预防作用是否也发生在培养的大鼠整个胚胎中。材料和方法：将孕10天的大鼠胚胎分别在正常培养液、高糖培养液、高糖培养液中添加多聚赖氨酸或L-精氨酸培养24 h，观察胚胎的生长发育情况。研究结果：L-精氨酸和腐胺部分防止高葡萄糖的畸形效应，而亚精胺和精胺几乎完全防止这些效应。结论：多胺直接保护胚胎免受高糖对生长发育的毒性影响，但其机制尚不清楚。出生缺陷研究（部分B）86：58-64，2009（C）2009 Wiley-Liss，Inc.
BACKGROUND: Pregnancy in mammals with diabetes mellitus results in low birth weight, malformations, and intrauterine death. Parenteral application of natural polyamines or their precursor, L-arginine, to diabetic pregnant rats partially prevents the alterations of development caused by diabetes mellitus. This experiment has been designed to understand if this preventive action also occurs in rat whole embryo in culture. MATERIALS AND METHODS: Rat embryos of gestational day 10 were cultured for 24h in normal medium,high glucose medium, or high glucose medium supplemented with poly mines or L-arginine, and furthermore embryo growth and development were evaluated. RESULTS: L-arginine and putrescine partially prevents the dysmorphogenic effects of high glucose, whereas spermidine and spermine prevent these effects almost completely. CONCLUSIONS: Polyamines directly protect the embryo from the toxic effect of high glucose concentration on growth and development, although the mechanism remains to be elucidated. Birth Defects Res (Part B) 86:58-64, 2009 (C) 2009 Wiley-Liss, Inc.