Radiation and chemically induced potentially lethal lesions in noncycling mammalian cells: recovery analysis in terms of x-ray- and ultraviolet-like-systems.
Radiation and chemically induced potentially lethal lesions in noncycling mammalian cells: recovery analysis in terms of x-ray- and ultraviolet-like-systems.
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辐射和化学诱导的非循环哺乳动物细胞潜在致命损伤：X 射线和类紫外线系统的恢复分析。
DOI：
10.2307/3574243
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发表时间：
1975
影响因子：
3.4
通讯作者：
G. Hahn
中科院分区：
文献类型：
--
作者：
G. Hahn
研究了中国仓鼠细胞暴露于紫外线和X射线照射后潜在致命性损伤的恢复和固定，以及暴露于化疗药物后的恢复。紫外线照射后的恢复有大约20小时的T/SUP$SUP 1$/$SUB 2$/；细胞能够进行恢复的比例取决于暴露前后的营养因素。在x射线照射后，恢复以大约2小时的T/SUB$SUP 1$/$SUB 2$/进行，并且受营养因素的影响要小得多。在紫外线照射的细胞中，血清刺激后的固定有3至4小时的T/sub1$/$sub2$/，在x射线照射的细胞中，T/sub1$/$sub2$/30分钟。氮芥末和博莱霉素暴露后的恢复动力学与X射线暴露后的恢复动力学相似；甲烷磺酸甲酯暴露后的恢复动力学主要是类紫外线的，尽管含有类似X射线的成分。被BUDR取代的DNA和可见光照射的细胞的恢复主要是类似X射线的，尽管有类似紫外线的成分。暴露于阿霉素或1，3-二(2-氯乙基)-1-亚硝脲的细胞不能恢复。
Recovery from and fixation of potentially lethal damage after exposure of Chinese hamster cells to uv and to x irradiation were investigated, as was recovery after exposure to chemotherapeutic agents. Recovery after uv radiation has a T/sub $sup 1$/$sub 2$/ of about 20 hr; the fraction of cells able to undergo recovery depends upon nutritional factors both before and after exposure. After x irradiation, recovery proceeds with a T/sub $sup 1$/$sub 2$/ of approximately 2 hr and is much less influenced by nutritional factors. Fixation after serum stimulation has a T/sub $sup 1$/$sub 2$/ of 3 to 4 hr in uv- irradiated cells, a T/sub $sup 1$/$sub 2$/ of 30 min in x-irradiated cells. Recovery kinetics after nitrogen mustard and bleomycin exposures mimic those for x-ray exposure; after methyl methane sulfonate the kinetics are mainly uv-like, though with an x-ray-like component. Recovery by cells with BUdR-substituted DNA and irradiated with visible light is primarily x-ray-like, though with a uv-like component. There is no recovery by cells exposed to adriamycin or to 1,3-bis(2- chloroethyl)-1-nitrosourea.