Selective electrode for dibenzyl dimethyl ammonium cation as indicator of the membrane potential in biological systems.
Selective electrode for dibenzyl dimethyl ammonium cation as indicator of the membrane potential in biological systems.
复制标题
二苄基二甲基铵阳离子的选择性电极作为生物系统中膜电位的指示剂。
DOI：
10.1016/0005-2736(77)90035-9
复制
发表时间：
1977
期刊：
影响因子：
--
通讯作者：
Y. Kobatake
中科院分区：
文献类型：
--
作者：
M. Muratsugu;Naoki Kamo;Kenzo Kurihara;Y. Kobatake
用四苯基硼酮（TPB−）包埋于二氯乙烷中，制备了对二苄基二甲基铵（DDA+）敏感的膜电位指示剂电极。对浓度在10− 2和3 · 10− 6 M之间的DDA+溶液，表现出快速和能斯特响应。在各种无机盐、ADP、ATP、可氧化底物和糖的存在下观察到对DDA+的高选择性。用该电极测定粪链球菌对DDA ~+的摄取，结果与Harold，F. M.和Papineau，D.（（1972）J. Membrane Biol.8，27-44和45-62）。虽然他们通过测量用离子交换剂处理的滤液的吸光度来确定介质中的DDA+浓度，但电极可以直接测量细菌悬浮液中的DDA+浓度而无需任何预处理。该电极还可以测量线粒体在缺氧过程中对DDA+的摄取。
The electrode sensitive to dibenzyl dimethyl ammonium (DDA+), which is considered to be an indicator of the membrane potential, was constructed by using tetraphenyl borone (TPB−) embedded in dichloroethane. Rapid and Nernstian responses were exhibited against DDA+solutions ranging between 10−2and 3 · 10−6M in concentration. High selectivity for DDA+was observed in the presence of various inorganic salts, ADP, ATP, oxidizable substrates and sugars. The electrode developed here was used to measure the DDA+uptake inStreptococcus faecalisand the results agreed with those reported by Harold, F.M. and Papineau, D. ((1972) J. Membrane Biol. 8, 27–44 and 45–62). While they determined the DDA+concentration in the medium by measuring the absorbance of the filtrate treated with the ion-exchangers, the electrode can measure directly the DDA+concentration in the bacterial suspension without any pretreatment. It was also shown that the electrode can measure the DDA+uptake in mitochondria during energization.