Analysis of training-induced changes in ethyl acetate odor maps using a new computational tool to map the glomerular layer of the olfactory bulb
Analysis of training-induced changes in ethyl acetate odor maps using a new computational tool to map the glomerular layer of the olfactory bulb
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DOI：
10.1093/chemse/bji055
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发表时间：
2005-09-01
期刊：
影响因子：
3.5
通讯作者：
Restrepo, D
中科院分区：
文献类型：
--
作者：
Salcedo, E;Zhang, CB;Restrepo, D
气味质量被认为是通过激活嗅球中部分重叠的肾小球子集（气味图）来编码的。小鼠遗传学研究表明，表达特定嗅觉受体的嗅觉感觉神经元（OSN）将其轴突靶向球中的少数个体肾小球。虽然OSN轴突的特异性靶向为气味图谱提供了分子基础，但功能图谱和分子图谱之间的关系仍有待了解。在这篇文章中，我们问的问题是否密集训练的小鼠在去/不去操作性条件反射气味辨别任务影响气味地图测定肾小球周围细胞c-fos的上调。数据分析是使用一套新开发的计算工具，旨在系统地映射成年小鼠嗅球肾小球的功能和分子特征。该套件提供了必要的工具来处理高分辨率的数字图像，地图标记的肾小球，可视化气味地图，并促进统计分析的模式，确定肾小球在嗅球。该软件根据肾小球活动、密度或面积生成气味图（密度图）。我们发现，训练上调的数量，成为c-fos阳性后，用乙酸乙酯刺激肾小球。
Odor quality is thought to be encoded by the activation of partially overlapping subsets of glomeruli in the olfactory bulb (odor maps). Mouse genetic studies have demonstrated that olfactory sensory neurons (OSNs) expressing a particular olfactory receptor target their axons to a few individual glomeruli in the bulb. While the specific targeting of OSN axons provides a molecular underpinning for the odor maps, much remains to be understood about the relationship between the functional and molecular maps. In this article, we ask the question whether intensive training of mice in a go/no-go operant conditioning odor discrimination task affects odor maps measured by determining c-fos up-regulation in periglomerular cells. Data analysis is performed using a newly developed suite of computational tools designed to systematically map functional and molecular features of glomeruli in the adult mouse olfactory bulb. This suite provides the necessary tools to process high-resolution digital images, map labeled glomeruli, visualize odor maps, and facilitate statistical analysis of patterns of identified glomeruli in the olfactory bulb. The software generates odor maps (density plots) based on glomerular activity, density, or area. We find that training up-regulates the number of glomeruli that become c-fos positive after stimulation with ethyl acetate.