Description of Mycobacterium conceptionense sp nov., a Mycobacterium fortuitum group organism isolated from a posttraumatic osteitis inflammation
Description of Mycobacterium conceptionense sp nov., a Mycobacterium fortuitum group organism isolated from a posttraumatic osteitis inflammation
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DOI：
10.1128/jcm.44.4.1268-1273.2006
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发表时间：
2006-04-01
影响因子：
9.4
通讯作者：
Drancourt, M
中科院分区：
文献类型：
--
作者：
Adékambi, T;Stein, A;Drancourt, M
从一名31岁女性患者的伤口液流出、骨组织活检和切除的皮肤组织中分离到一种无色素的快速生长的分枝杆菌，该女性患者因意外发生右侧胫骨开放性骨折并长期呆在河里。这三个分离株在24~37℃下生长3天，16S rRNA序列分析表明，它们与关系最密切的物种--分枝杆菌的16S rRNA序列完全相同，序列相似性为99.7%(相差4个碱基)。RpoB(723bp)部分序列分析表明，分离株与猪分枝杆菌的rpoB基因序列相似性为97.0%。进一步的多相方法，包括生化试验、药敏分析、hsp65、sodA4和recA基因序列分析，以及%G+C测定和细胞壁脂肪酸组成分析，支持这些分离物代表一个新物种。系统发育分析表明，偶发分枝杆菌与门静脉分枝杆菌有较近的亲缘关系。这些菌株对大多数抗生素敏感，并显示出青霉素酶活性，这与门静脉分枝杆菌相反。我们建议将其命名为概念分枝杆菌。11月这一新物种与创伤后骨炎有关。模式毒株为D16(T)(相当于CIP 108544(T)和CCUG 50187(T))。
A nonpigmented rapidly growing mycobacterium was isolated from wound liquid outflow, bone tissue biopsy, and excised skin tissue from a 31-year-old woman who suffered an accidental open right tibia fracture and prolonged stay in a river. The three isolates grew in 3 days at 24 to 37 degrees C. 16S rRNA sequence analyses over 1,483 bp showed that they were identical and shared 99.7% (4-bp difference) sequence similarity with that of Mycobacterium porcinum, the most closely related species. Partial rpoB (723 bp) sequence analyses showed that the isolates shared 97.0% sequence similarity with that of M. porcinum. Further polyphasic approaches, including biochemical tests, antimicrobial susceptibility analyses, and hsp65, sodA4, and recA gene sequence analysis, as well as % G+C determination and cell wall fatty acid composition analysis supported the evidence that these isolates were representative of a new species. Phylogenetic analyses showed the close relationship with M. porcinum in the Mycobacterium fortuitum group. The isolates were susceptible to most antibiotics and exhibited evidence for penicillinase activity, in contrast to M. porcinum. We pro-pose the name Mycobacterium conceptionense sp. nov. for this new species associated with posttraumatic osteitis. The type strain is D16(T) (equivalent to CIP 108544(T) and CCUG 50187(T)).