Defining the antibody cross-reactome directed against the influenza virus surface glycoproteins.
Defining the antibody cross-reactome directed against the influenza virus surface glycoproteins.
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DOI：
10.1038/ni.3684
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发表时间：
2017-04
影响因子：
30.5
通讯作者：
Krammer F
中科院分区：
文献类型：
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作者：
Nachbagauer R;Choi A;Hirsh A;Margine I;Iida S;Barrera A;Ferres M;Albrecht RA;García-Sastre A;Bouvier NM;Ito K;Medina RA;Palese P;Krammer F
流感病毒感染可诱导针对病毒表面糖蛋白血凝素和神经氨酸酶的抗体，这些反应具有广泛的保护性。为了测试抗体反应的广度和幅度，小鼠、豚鼠和雪貂依次感染了不同的H1N1或H3N2病毒。抗体反应通过酶联免疫吸附试验对代表病毒多样性的一组重组糖蛋白进行检测。豚鼠产生了高滴度的广泛交叉反应抗体；小鼠和雪貂表现出较窄的体液反应。然后，我们比较了人类感染H1N1或H3N2后的抗体反应，发现了明显广泛的反应和原始抗原性SIN的令人信服的证据。这项工作将为通用流感疫苗的设计提供信息，并可以指导针对新出现的流感病毒的大流行准备工作。
Influenza virus infections induce antibodies against the viral surface glycoproteins hemagglutinin and neuraminidase, and these responses can be broadly protective. To test the breadth and magnitude of antibody responses, mice, guinea pigs and ferrets were sequentially infected with divergent H1N1 or H3N2 viruses. Antibody responses were measured by ELISA against an extensive panel of recombinant glycoproteins representing the viral diversity in nature. Guinea pigs developed high titers of broadly cross-reactive antibodies; mice and ferrets exhibited narrower humoral responses. Then, we compared antibody responses after H1N1 or H3N2 infections in humans and found markedly broad responses and cogent evidence for original antigenic sin. This work will inform universal influenza vaccine design and can guide pandemic preparedness efforts against emerging influenza viruses.