Electrical characteristics of rat skeletal muscle in immaturity, adulthood and after sciatic nerve injury, and their relation to muscle fiber size
Electrical characteristics of rat skeletal muscle in immaturity, adulthood and after sciatic nerve injury, and their relation to muscle fiber size
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DOI：
10.1088/0967-3334/30/12/009
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发表时间：
2009-12-01
影响因子：
3.2
通讯作者：
Rutkove, Seward B.
中科院分区：
文献类型：
--
作者：
Ahad, Mohammad A.;Fogerson, P. Michelle;Rutkove, Seward B.
局部阻抗法可为评估神经肌肉疾病提供有用的方法。然而，这些阻抗变化的机制仍然不确定。为了开始了解肌肉病理与表面阻抗值的关系，处死了8只未成年大鼠、12只成年大鼠和8只经历了坐骨挤压的成年大鼠。在阻抗单元中对来自每只动物的腓肠肌的组织进行测量，并且在2kHz至1MHz的频率下计算组织在纵向和横向两个方向上的电导率和相对介电常数。此外，对组织进行定量组织学分析。横向电导率和横向相对介电常数的显着升高，发现与动物的增长，但纵向值没有表现出差异。坐骨挤压后，横向和纵向电导率显着增加，在任何方向上的相对介电常数没有变化。神经损伤后，这些值的频率依赖性也发生了变化。在健康动物中，测得的电导率和相对介电常数与细胞面积之间有很强的线性关系，但不为坐骨神经挤压动物。这些结果为全面了解肌肉电特性如何在神经肌肉疾病状态下改变迈出了第一步。
Localized impedance methods can provide useful approaches for assessing neuromuscular disease. The mechanism of these impedance changes remains, however, uncertain. In order to begin to understand the relation of muscle pathology to surface impedance values, 8 immature rats, 12 mature rats and 8 mature rats that had undergone sciatic crush were killed. Measurement was made on tissue from the gastrocnemius muscle from each animal in an impedance cell, and the conductivity and relative permittivity of the tissue were calculated in both the longitudinal and transverse directions for frequencies of 2 kHz to 1 MHz. In addition, quantitative histological analysis was performed on the tissue. Significant elevations in transverse conductivity and transverse relative permittivity were found with animal growth, but longitudinal values showed no difference. After sciatic crush, both transverse and longitudinal conductivity increased significantly, with no change in the relative permittivity in either direction. The frequency dependence of the values also changed after nerve injury. In the healthy animals, there was a strong linear relation between measured conductivity and relative permittivity with cell area, but not for the sciatic crush animals. These results provide a first step toward developing a comprehensive understanding of how the electrical properties of muscle alter in neuromuscular disease states.