Real-time reverse transcriptase PCR assay for detection of human metapneumoviruses from all known genetic lineages
Real-time reverse transcriptase PCR assay for detection of human metapneumoviruses from all known genetic lineages
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DOI：
10.1128/jcm.42.3.981-986.2004
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发表时间：
2004-03-01
影响因子：
9.4
通讯作者：
Fouchier, RAM
中科院分区：
文献类型：
--
作者：
Maertzdorf, J;Wang, CK;Fouchier, RAM
人类偏肺病毒的发现及其对呼吸道疾病的影响强调需要一种灵敏、特异和快速的检测方法来在临床环境中检测这种病毒。最近人们清楚，人类偏肺病毒可以分为至少四个遗传谱系。先前描述的人类偏肺病毒检测方法是通过使用有限的序列信息开发的，并且未能以相当的灵敏度检测来自所有四种遗传谱系的病毒。在这里，我们描述了实时逆转录酶 PCR 检测的开发和评估，该检测以相同的特异性和敏感性从四种已知的遗传谱系中检测人类偏肺病毒。
The discovery of human metapneumovirus and its implications for respiratory tract disease have emphasized the need for a sensitive, specific, and rapid assay to detect this virus in a clinical setting. It recently became clear that human metapneumovirus can be grouped into at least four genetic lineages. Previously described assays for the detection of human metapneumovirus were developed by using limited sequence information and failed to detect viruses from all four genetic lineages with comparable sensitivities. Here we describe the development and evaluation of a real-time reverse transcriptase PCR assay that detects human metapneumovirus from the four known genetic lineages with equal specificities and sensitivities.