Contrasting alteration patterns of different cartilage plates in knee articular cartilage after spinal cord injury in rats
Contrasting alteration patterns of different cartilage plates in knee articular cartilage after spinal cord injury in rats
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DOI：
10.1038/sc.2008.96
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发表时间：
2009-03
期刊：
影响因子：
2.2
通讯作者：
H. Moriyama;K. Nishihara;M. Hosoda;Y. Saka;N. Kanemura;K. Takayanagi;O. Yoshimura;Y. Tobimatsu
中科院分区：
文献类型：
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作者：
H. Moriyama;K. Nishihara;M. Hosoda;Y. Saka;N. Kanemura;K. Takayanagi;O. Yoshimura;Y. Tobimatsu
研究设计：实验，对照试验，动物研究。目的：评估脊髓损伤后不同软骨板的形态学变化，并确定这些变化的定位。设置：琦玉，日本。方法：共使用16只Wistar大鼠。8只大鼠进行脊髓损伤，8只大鼠没有干预作为对照。通过X线片和组织形态计量学分析评价膝关节软骨的改变。为了量化软骨的变化，我们选择的组织学特征：关节软骨的厚度，软骨细胞的数量，甲苯胺蓝基质染色作为蛋白多糖含量和表面irregularity.Results的反映：在膝关节之间没有差异，发现通过X射线摄影。在内侧膝关节中，脊髓损伤膝关节的软骨厚度在股骨前部区域增加，在胫骨和股骨后部区域减少;然而，在外侧膝关节中，脊髓损伤膝关节的软骨厚度与对照膝关节相比没有变化。脊髓损伤后软骨细胞数量减少，尤其是在股骨前区。胫骨区域的基质染色部分增加。脊髓损伤膝关节表面的不规则性与对照膝关节的所有软骨板。结论：目前的研究结果显示脊髓损伤后的软骨的特点。这些改变在内侧和外侧区域之间的性质不同。未来的研究应该分别评估不同的软骨板，当检查内侧膝关节的变化时高估这些严重性，而当检查外侧膝关节的变化时低估这些严重性。
Study design:Experimental, controlled trial, animal study.Objective:To assess morphologic changes in different cartilage plates after spinal cord injury and identify the localization of these alterations.Setting:Saitama, Japan.Methods:A total of 16 Wistar rats were used. Eight rats underwent a spinal cord injury and eight rats had no intervention as control. The cartilage alterations of the knee joint were evaluated with radiography and histomorphometric analysis. To quantify cartilage alterations, we selected the histologic characteristics: thickness of the articular cartilage, number of chondrocytes, matrix staining to toluidine blue as a reflection of proteoglycan content and surface irregularity.Results:No differences in knee joints were found between the groups by radiography. In the medial knee joint, cartilage thickness of spinal-cord-injured knees increased at the anterior femoral region and decreased at the tibial and posterior femoral regions; however, in the lateral knee, that of spinal cord injuries did not change compared with control knees. Spinal cord injuries decreased the number of chondrocytes, especially at the anterior femoral regions. Matrix staining increased partially at the tibial regions. Surface irregularity of spinal-cord-injured knees was comparable to that of control knees in all cartilage plates.Conclusion:The present findings exhibit characteristics of the cartilage after spinal cord injury. These alterations were different in nature between the medial and lateral regions. Future studies should assess separately different cartilage plates, to overestimate these severities when the changes at the medial knee were examined and to underestimate when the changes at the lateral knee were examined.