Effects of autonomic denervations on the rhythms in axial length and choroidal thickness in chicks.
Effects of autonomic denervations on the rhythms in axial length and choroidal thickness in chicks.
复制标题
自主神经去神经对雏鸡眼轴长度和脉络膜厚度节律的影响。
DOI：
10.1007/s00359-018-01310-4
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发表时间：
2019
期刊：
影响因子：
--
通讯作者：
Schroedl,Falk
中科院分区：
文献类型：
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作者：
Nickla,DeboraL;Schroedl,Falk
在雏鸡中，眼轴长度和脉络膜厚度经历昼夜节律振荡。脉络膜受自主神经系统的两个分支支配，但它们对这些节律的贡献尚不清楚。我们使用两个组合病变来测试这一点。对于副交感神经切除术，将神经 VII 从突触前切至翼腭神经节，并切断睫状神经节后（双重损伤；n= 8）。三重病变还切除了交感颈上神经节（n= 8）。对照组进行假手术（n=≥7）。 8-14 天后，在 24 小时内每隔 4 小时通过超声检查测量轴向尺寸。使用“最佳拟合”功能评估节律参数，并测量生长率。两种类型的病变都会导致脉络膜厚度和眼轴长度出现超昼夜节律（> 1个周期/24小时），并增加玻璃体腔生长（Exp-fellow：双倍：69 µm；三倍：104 µm；p< 0.05）。对于双病变，两种节律的频率均为 1.5 个周期/天；对于三胞胎，脉络膜节律为 1.5 个周期/天，轴向节律为 3 个周期/天。对于双病变，两种节律的振幅均大于假手术对照（轴向：107 vs 54 µm；脉络膜：124 vs 29 µm，p< 0.05）。这些发现为异常眼节律参与近视发展的生长刺激提供了证据。
In chicks, axial length and choroidal thickness undergo circadian oscillations. The choroid is innervated by both branches of the autonomic nervous system, but their contribution(s) to these rhythms is unknown. We used two combination lesions to test this. For parasympathectomy, nerve VII was sectioned presynaptic to the pterygopalatine ganglia, and the ciliary post-ganglionics were cut (double lesion;n= 8). Triple lesions excised the sympathetic superior cervical ganglion as well (n= 8). Sham surgery was done in controls (n= 7). 8–14 days later, axial dimensions were measured with ultrasonography at 4-h intervals over 24 h. Rhythm parameters were assessed using a “best fit” function, and growth rates measured. Both types of lesions resulted in ultradian (> 1 cycle/24 h) rhythms in choroidal thickness and axial length, and increased vitreous chamber growth (Exp-fellow: double: 69 µm; triple: 104 µm;p< 0.05). For double lesions, the frequency was 1.5 cycles/day for both rhythms; for triples the choroidal rhythm was 1.5 cycles/day, and the axial was 3 cycles/day. For double lesions, the amplitudes of both rhythms were larger than those of sham surgery controls (axial: 107 vs 54 µm; choroid: 124 vs 29 µm,p< 0.05). These findings provide evidence for the involvement of abnormal ocular rhythms in the growth stimulation underlying myopia development.