Population structure of the Japanese eel Anguilla japonica as examined by mitochondrial DNA sequencing
Population structure of the Japanese eel Anguilla japonica as examined by mitochondrial DNA sequencing
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DOI：
10.1046/j.1444-2906.2001.00227.x
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发表时间：
2001-04-01
影响因子：
1.9
通讯作者：
Nishida, M
中科院分区：
文献类型：
--
作者：
Ishikawa, S;Aoyama, J;Nishida, M
为了研究日本鳗鲡的种群结构，线粒体DNA序列分析的各种样品的玻璃鳗鱼，可能反映产卵聚集体的遗传特征。对采自种子岛、神奈川和茨木的51条玻璃鳗的线粒体DNA进行了测序，得到了从tRNA（Thr）基因到控制区中部的615个碱基对的片段。DNA区域是如此多变，以至于没有发现个体具有相同的序列，尽管样品内的平均序列差异（1.07-1.63）并不大。样品间的平均序列差异（1.22-1.57）与样品内的序列差异相当，表明样品间无遗传异质性。序列的树分析既没有显示种群的地理结构，也没有显示种群的时间结构。此外，尽管本研究的所有DNA序列彼此不同，但发现两个序列与不同年份从不同地点收集的个体报告的序列相同。总的来说，目前的线粒体DNA序列分析的玻璃鳗鱼没有提供任何证据的遗传分化的日本鳗鱼种群。
To examine the population structure of the Japanese eel Anguilla japonica, mitochondrial DNA sequence analysis was made for various samples of glass eels that might reflect genetic characteristics of spawning aggregates. The mitochondrial DNA from a total of 51 glass eels collected at Tanegashima Island, Kanagawa and Ibaraki in different periods within an inshore migrating season was sequenced for a 615 base-pair fragment from the tRNA(Thr) gene to the central part of the control region. The DNA region was so variable that no individual was found to possess the same sequence, although average sequence differences within samples (1.07-1.63) were not large. Average sequence differences between samples (1.22-1.57) were comparable to those within samples, suggesting no genetic heterogeneity among samples. Tree analysis of the sequences showed neither a geographical nor temporal structure of population. Furthermore, although all DNA sequences from the present study were different from one another, two sequences were found to be the same with those reported from individuals collected in different localities in different years. Altogether, the present mitochondrial DNA sequence analysis of glass eels did not provide any evidence for genetic subdivision of the Japanese eel population.