Cloning of Six ERF Family Transcription Factor Genes from Peanut and Analysis of their Expression during Abiotic Stress
Cloning of Six ERF Family Transcription Factor Genes from Peanut and Analysis of their Expression during Abiotic Stress
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DOI：
10.1007/s11105-012-0456-0
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发表时间：
2012-12-01
影响因子：
2.1
通讯作者：
Yu, Shanlin
中科院分区：
文献类型：
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作者：
Chen, Na;Yang, Qingli;Yu, Shanlin
几种乙烯响应元件结合因子 (ERF) 家族蛋白已被证明在多种生物过程的转录调节中发挥重要作用，包括对干旱、盐和寒冷等环境条件的响应。迄今为止，花生中尚未见该科的报道。本研究在花生cDNA文库中鉴定出40个EST，其氨基酸序列含有AP2/ERF结构域。其中，克隆了六个基因的全长序列。序列分析表明，所有六种蛋白均仅含有一个AP2/ERF结构域，应属于ERF家族。这六个基因被命名为花生 ERF 1-6 (AhERF1-6)。分析了冷、盐和干旱胁迫下 AhERF1-6 的表达模式。结果表明，AhERF4和AhERF6的表达在非生物胁迫下迅速且大幅增强。 AhERF1和AhEERF5的表达在一定胁迫条件下略有增强。一些基因在胁迫下下调，例如盐胁迫下叶片中的AhEERF3和干旱胁迫下叶片中的AhERF2。有趣的是，在 PEG 处理后，AhERF3 和 AhERF5 在花生叶和根中的表达表现出相反的表达模式。这些结果表明不同的ERF蛋白在花生非生物胁迫驯化中可能具有不同的功能。
Several ethylene-responsive element binding factor (ERF) family proteins have been demonstrated to play important roles in transcriptional regulation of a variety of biological processes, including responses to environmental conditions such as drought, salt, and cold. To date, there have been no reports on this family in peanut. In this study, 40 ESTs in a peanut cDNA library, whose amino acid sequences contained AP2/ ERF domain, have been identified. Of these, full-length sequences of six genes were cloned. Sequence analysis indicated that all six proteins contained only one AP2/ ERF domain and should belong to the ERF family. The six genes were designated Arachis hypogaea ERF 1-6 (AhERF1-6). The expression patterns of AhERF1-6 were analyzed under cold, salt and drought stress. The results indicated that the expression of AhERF4 and AhERF6 were rapidly and substantially enhanced under abiotic stress. The expression of AhERF1 and AhEERF5 were slightly enhanced under certain stress conditions. Some genes were down-regulated when under stress, such as AhEERF3 in leaves under salt stress and AhERF2 in leaves under drought stress. Interestingly, the expression of AhERF3 and AhERF5 exhibited contrary expression patterns in peanut leaves and roots upon PEG treatment. These results suggested that different ERF proteins may have different functions in peanut abiotic stress acclimation.