Blood compatibility of surfaces with superlow protein adsorption.
Blood compatibility of surfaces with superlow protein adsorption.
复制标题
DOI：
10.1016/j.biomaterials.2008.07.039
复制
发表时间：
2008-11
期刊：
影响因子：
14
通讯作者：
Zheng Zhang;Min Zhang;Shengfu Chen;T. Horbett;B. Ratner;Shaoyi Jiang
中科院分区：
文献类型：
--
作者：
Zheng Zhang;Min Zhang;Shengfu Chen;T. Horbett;B. Ratner;Shaoyi Jiang
在这项工作中，制备了五个自组装单层（SAM）和三个具有非常低纤维蛋白原吸附的聚合物刷。五个 SAM 是低聚乙二醇 (OEG)、磷酸胆碱 (PC)、低聚磷酸胆碱 (OPC) 以及 SO3−/N+(CH3)3(SA/TMA) 和 COO−/N+(CH3)3(CA/TMA) 两种带正电和负电的混合 SAM。使用磺基甜菜碱甲基丙烯酸酯 (SBMA)、羧基甜菜碱甲基丙烯酸酯 (CBMA) 和低聚乙二醇甲基醚甲基丙烯酸酯 (OEGMA) 三种单体，通过表面引发原子转移自由基聚合 (ATRP) 在金表面制备三个聚合物刷。表面等离子共振 (SPR) 测量表明，尽管所有这些表面对于缓冲溶液中的纤维蛋白原吸附都是“无污染”的，但它们对未稀释的人血浆中的蛋白质吸附差异很大。与所测试的五种 SAM 中的任何一种相比，聚合物刷对血浆的蛋白质吸附量要低得多。然而，血浆预吸附表面上的血小板粘附测量表明，所有这些表面都具有非常低的血小板粘附。使用再钙化贫血小板血浆 (PPP) 与八种类型的表面孵育进行的凝血时间测量表明，它们不会缩短凝血时间。还合成并表征了具有相似分子量的聚SBMA和聚CBMA的线性聚合物。在聚CBMA线性聚合物存在下，PPP的凝血时间延长并随着聚合物浓度的增加而增加，而聚SBMA或PEG聚合物没有观察到抗凝血活性。 PolyCBMA 独特的抗凝活性及其高血浆蛋白吸附性，使 PolyCBMA 成为血液接触应用的候选者。
In this work, five self-assembled monolayers (SAMs) and three polymeric brushes with very low fibrinogen adsorption were prepared. The five SAMs are oligo(ethylene glycol) (OEG), phosphorylcholine (PC), oligo(phosphorylcholine) (OPC), and two mixed positively and negatively charged SAMs of SO3−/N+(CH3)3(SA/TMA) and COO−/N+(CH3)3(CA/TMA). Three polymer brushes were prepared on gold surfaces via surface-initiated atom transfer radical polymerization (ATRP) using three monomers, sulfobetaine methacrylate (SBMA), carboxybetaine methacrylate (CBMA), and oligo(ethylene glycol) methyl ether methacrylate (OEGMA). Surface plasmon resonance (SPR) measurements show that although all of these surfaces are “nonfouling” to fibrinogen adsorption from buffer solution, their protein adsorption from undiluted human blood plasma varies widely. Polymer brushes exhibit much lower protein adsorption from plasma than any of the five SAMs tested. However, platelet adhesion measurements on plasma-preadsorbed surfaces show that all of these surfaces have very low platelet adhesion. Clotting time measurements using recalcified platelet poor plasma (PPP) incubation with the eight types of surfaces show that they do not shorten clotting times. Linear polymers of polySBMA and polyCBMA with similar molecular weights were also synthesized and characterized. In the presence of polyCBMA linear polymers, the clotting time of PPP was prolonged and increased with the concentration of the polymer, while no anticoagulant activity was observed for the polySBMA or PEG polymers. The unique anticoagulant activity of polyCBMA, as well as its high plasma protein adsorption resistance, makes polyCBMA a candidate for blood-contacting applications.