Genetic Analysis of Selected Strains of Eastern Oyster (Crassostrea virginica Gmelin) Using AFLP and Microsatellite Markers
Genetic Analysis of Selected Strains of Eastern Oyster (Crassostrea virginica Gmelin) Using AFLP and Microsatellite Markers
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DOI：
10.1007/s10126-004-3600-5
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发表时间：
2004-11
影响因子：
3
通讯作者：
Ziniu Yu;Ximing Guo
中科院分区：
文献类型：
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作者：
Ziniu Yu;Ximing Guo
利用AFLP和微卫星标记技术，对4个东方牡蛎品系（DBH、NEH、FMF和CTS）和1个野生东方牡蛎群体（DBW）的遗传变异和分化进行了研究。利用来自3对引物的86个AFLP标记和5个微卫星标记对5个群体的30个牡蛎进行了分析。微卫星位点的变异性比AFLP大得多。观察到的杂合度范围从0.560到0.640的微卫星和从0.186到0.207的AFLP各群体。微卫星数据的F和φ PT以及AFLP数据的φ PT统计量均表明，所有对群体之间存在显著的分化。在所有4个选择的菌株中杂合性没有显着减少，但是，每个位点的等位基因数在选择的菌株中比在野生种群中低得多。经过长期抗病选择的两个菌株在一些位点上发生了频率偏移，这值得进一步分析，以确定它们是否与抗病基因连锁。
Amplified fragment length polymorphisms (AFLPs) and microsatellite markers were used to examine genetic variation and divergence in 4 selected strains (DBH, NEH, FMF, and CTS) and 1 wild population (DBW) of the eastern oysterCrassostrea virginicaGmelin. Eighty-six AFLP markers (from 3 primer pairs) and 5 microsatellite loci were used for the analysis of 30 oysters from each of the 5 populations. Microsatellite loci were considerably more variable than AFLPs. The observed heterozygosity ranged from 0.560 to 0.640 across populations for microsatellites, and from 0.186 to 0.207 for AFLPs. BothFstand φPTof microsatellite data and φPTstatistics of AFLP data revealed significant divergence between all pairs of populations. There was no significant reduction in heterozygosity in all 4 selected strains; however, the number of alleles per locus was considerably lower in the selected strains than in the wild population. Two strains subjected to long-term selection for disease resistance shared frequency shifts at a few loci, which deserve further analysis to determine if they are linked to disease-resistance genes.