Functional analysis of an APETALA1-like MADS box gene from Eustoma grandiflorum in regulating floral transition and formation
Functional analysis of an APETALA1-like MADS box gene from Eustoma grandiflorum in regulating floral transition and formation
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DOI：
10.1007/s11816-018-0475-x
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发表时间：
2018-02
影响因子：
2.4
通讯作者：
T. Chuang;Kun-Hung Li;Pei-Fang Li;Chang-Hsien Yang
中科院分区：
文献类型：
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作者：
T. Chuang;Kun-Hung Li;Pei-Fang Li;Chang-Hsien Yang
分离并鉴定了与MADS-box准亚家族高度同源的桔梗aptala1 （EgAP1）基因。EgAP1在c端含有一个保守的euap1基序，与antirrhinum majus squamosin the quassubfamily具有高度的序列同源性。EgAP1mRNA在叶片中检测到，且在幼花蕾中的表达量显著高于成熟花蕾。在花中，EgAP1mRNA在萼片中表达强烈，在花瓣中表达弱，在雄蕊和心皮中不存在。异位表达egap1的转基因拟南芥植株开花早，产生顶花。此外，在35S:: egap1花中也观察到花瓣向雄蕊样结构的转化。在拟南芥ap1突变体植株中，egap1能够通过修复萼片形成缺陷和显著提高第二轮花瓣产量来弥补ap1花缺陷。这些结果表明，ategap1是apetala1的同源物。fegap1的功能参与了花的诱导和花的形成。
AnEustoma grandiflorum APETALA1 (EgAP1)gene showing high homology to theSQUAsubfamily of MADS-box genes was isolated and characterized.EgAP1, containing a conserved euAP1motif at the C-terminus, showed high sequence identity toAntirrhinum majus SQUAMOSAin theSQUAsubfamily.EgAP1mRNA was detected in the leaf and expressed significantly higher in young flower buds than in mature flower buds. In flowers,EgAP1mRNA was strongly detected in sepal, weakly detected in petal and was absent in stamen and carpel. Transgenic Arabidopsis plants ectopically expressingEgAP1flowered early and produced terminal flowers. In addition, the conversion of petals into stamen-like structures was also observed in 35S::EgAP1flowers. 35S::EgAP1was able to complement theap1flower defects by restoring the defect for sepal formation and significantly increasing second whorl petal production inArabidopsis ap1mutant plants. These results revealed thatEgAP1is theAPETALA1homolog inE. grandiflorumand that the function ofEgAP1is involved in floral induction and flower formation.