Identification of the rrmA gene encoding the 23S rRNA m1G745 methyltransferase in Escherichia coli and characterization of an m1G745-deficient mutant
Identification of the rrmA gene encoding the 23S rRNA m1G745 methyltransferase in Escherichia coli and characterization of an m1G745-deficient mutant
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DOI：
10.1128/jb.180.2.359-365.1998
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发表时间：
1998-01-01
影响因子：
3.2
通讯作者：
Persson, BC
中科院分区：
文献类型：
--
作者：
Gustafsson, C;Persson, BC
以前已经分离到一个在rRNA中缺乏修饰核苷酸m(1)G的大肠杆菌突变体(G.R.Bjork和L.A.Isaksson，J.Mel)。比奥尔。51：83-100,1970)。在这项研究中，我们定位了23S rRNA中m(1)G到核苷酸745的位置，并鉴定了这一修饰缺陷的突变体。该突变株在富营养介质中的生长速度下降了40%，松散耦合的核糖体急剧减少，多肽链延长率下降了20%，对核糖体结合的抗生素维霉素的耐药性增加。将编码23S rRNA m(1)G745甲基转移酶的rrmA基因定位于大肠杆菌染色体上的1904000个碱基上，经鉴定与先前测序的基因yebH完全相同。
An Escherichia coli mutant lacking the modified nucleotide m(1)G in rRNA has previously been isolated (G. R. Bjork and L. A. Isaksson, J. Mel. Biol. 51:83-100, 1970). In this study, we localize the position of the m(1)G to nucleotide 745 in 23S rRNA and characterize a mutant deficient in this modification. This mutant shows a 40% decreased growth rate in rich media, a drastic reduction in loosely coupled ribosomes, a 20% decreased polypeptide chain elongation rate, and increased resistance to the ribosome binding antibiotic viomycin. The rrmA gene encoding 23S rRNA m(1)G745 methyltransferase was mapped to bp 1904000 on the E. coli chromosome and identified to be identical to the previously sequenced gene yebH.