Structural and functional partitioning of bread wheat chromosome 3B
Structural and functional partitioning of bread wheat chromosome 3B
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DOI：
10.1126/science.1249721
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发表时间：
2014-07-18
期刊：
影响因子：
56.9
通讯作者：
Feuillet, Catherine
中科院分区：
文献类型：
--
作者：
Choulet, Frederic;Alberti, Adriana;Feuillet, Catherine
我们制作了一个六倍体面包小麦的10亿染色体3B的参考序列。通过对8452条细菌人工染色体进行测序，我们组装了一个774兆碱基的序列，携带5326个蛋白质编码基因，1938个假基因和85%的转座因子。结构和功能特征的分布沿着染色体显示分区与减数分裂重组。比较分析表明，高小麦特异性染色体间和染色体内基因复制活动的适应变异的潜在来源。除了更好地理解大型多倍体基因组的组织、功能和进化外，锚定在遗传图谱上的高质量序列的可用性将加速重要农艺性状基因的鉴定。
We produced a reference sequence of the 1-gigabase chromosome 3B of hexaploid bread wheat. By sequencing 8452 bacterial artificial chromosomes in pools, we assembled a sequence of 774 megabases carrying 5326 protein-coding genes, 1938 pseudogenes, and 85% of transposable elements. The distribution of structural and functional features along the chromosome revealed partitioning correlated with meiotic recombination. Comparative analyses indicated high wheat-specific inter-and intrachromosomal gene duplication activities that are potential sources of variability for adaption. In addition to providing a better understanding of the organization, function, and evolution of a large and polyploid genome, the availability of a high-quality sequence anchored to genetic maps will accelerate the identification of genes underlying important agronomic traits.