Characterization of the Toll-like receptor expression profile in human multiple myeloma cells.
Characterization of the Toll-like receptor expression profile in human multiple myeloma cells.
复制标题
DOI：
10.1371/journal.pone.0060671
复制
发表时间：
2013
期刊：
影响因子：
3.7
通讯作者：
Redegeld F
中科院分区：
文献类型：
--
作者：
Abdi J;Mutis T;Garssen J;Redegeld F
Toll样受体(TLRs)在多发性骨髓瘤(MM)中的表达和功能已成为近年来研究的热点。了解这些受体在MM中的表达和生物学特性将使我们对炎症环境在MM疾病进展或发病机制中的作用有一个新的认识。然而，到目前为止，TLR在MM中的表达模式是非常不同的，特别是在基因水平上，而关于TLR在蛋白质水平上的表达的信息很少。在本研究中，我们从基因和蛋白水平检测了人骨髓瘤细胞系Fravel、L363、UM6、UM9、OPM1、OPM2、U266、RPMI8226、XG1和NCI H929以及MM患者原代细胞中TLR的表达。我们发现所有细胞系和原代细胞都表达TLR1、TLR3、TLR4、TLR7、TLR8和TLR9的mRNA和蛋白。TLR2和TLR5在大多数HMCL的mRNA水平上有表达，但在蛋白水平上检测不到，而原始样本中TLR2、TLR3和TLR5的蛋白表达水平较低。我们的结果表明，MM细胞表达广泛的TLR，但在基因和蛋白质表达模式之间存在一定程度的差异。TLRs在多发性骨髓瘤细胞中的明确表达表明其对肿瘤诱导的炎症信号有反应的倾向，这在多发性骨髓瘤的骨髓环境中似乎是不可避免的。
Expression and function of Toll-like receptors (TLRs) in multiple myeloma (MM) has recently become the focus of several studies. Knowledge of expression and biology of these receptors in MM will provide us with a new insight into the role of an inflammatory environment in disease progression or pathogenesis of MM. However, to date a quite heterogeneous expression pattern of TLRs in MM particularly at gene level has been described while information on the TLR expression at the protein level is largely unavailable. In this study, we investigated the TLR expression in human myeloma cell lines (HMCLs) Fravel, L363, UM6, UM9, OPM1, OPM2, U266, RPMI 8226, XG1, and NCI H929 and primary cells from MM patients at both mRNA and protein level (western blot and flow cytometry). We found that all cell lines and primary cells expressed TLR1, TLR3, TLR4, TLR7, TLR8, and TLR9 mRNA and protein. TLR2 and TLR5 were expressed by the majority of HMCLs at mRNA but were not detectable at protein level, while primary samples showed a low level of TLR2, TLR3 and TLR5 protein expression. Our results indicate that MM cells express a broad range of TLRs with a degree of disparity between gene and protein expression pattern. The clear expression of TLRs in MM cells indicates a propensity for responding to tumor-induced inflammatory signals, which seem inevitable in the MM bone marrow environment.
登录
查看更多内容
影响因子：
11.4
作者：
Jego, G;Bataille, R;Pellat-Deceunynck, C
通讯作者：
Pellat-Deceunynck, C
影响因子：
11.4
作者：
van de Donk, NWCJ;Kamphuis, MMJ;Bloem, AC
通讯作者：
Bloem, AC
影响因子：
6.5
作者：
Xu, Yang;Zhao, Yun;Wu, Depei
通讯作者：
Wu, Depei
DOI：
10.3324/haematol.2010.030759
发表时间：
2011-02-01
期刊：
HAEMATOLOGICA-THE HEMATOLOGY JOURNAL
影响因子：
--
作者：
van der Veer, Michael. S.;de Weers, Michel;Mutis, Tuna
通讯作者：
Mutis, Tuna
影响因子：
11.4
作者：
Bohnhorst, J;Rasmussen, T;Sundan, A
通讯作者：
Sundan, A