Immortalisation of human urothelial cells
Immortalisation of human urothelial cells
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DOI：
10.1007/bf00698738
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发表时间：
1995-12-01
影响因子：
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通讯作者：
Masters, JRW
中科院分区：
文献类型：
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作者：
Petzoldt, JL;Leigh, IM;Masters, JRW
使用温度敏感的SV40大T抗原基因构建体使源自12岁女孩输尿管衬里的尿路上皮的细胞系永生化，并命名为UROtsa。永生化后，UROtsa细胞表达SV40大T抗原，但没有获得肿瘤转化的特征，包括在软琼脂中生长或在裸鼠中发生肿瘤。中期涂片的染色体外观和数量正常。UROtsa细胞在39摄氏度下仍然允许细胞生长，表明它们没有保持温度敏感性。UROtsa提供了一种“正常"尿路刺激的体外模型。
A cell line derived from the urothelium lining the ureter of a 12-year-old girl was immortalised using a temperature-sensitive SV40 large T-antigen gene construct, and designated UROtsa. Following immortalisation, UROtsa cells expressed SV40 large T-antigen, but did not acquire characteristics of neoplastic transformation, including growth in soft agar or the development of tumours in nude mice. Metaphase spreads had a normal chromosomal appearance and number. UROtsa cells remained permissive for cell growth at 39 degrees C, indicating that they did not retain temperature sensitivity. UROtsa provides an in vitro model of ''normal'' urothelium.