NITRILASE-CATALYZED PRODUCTION OF NICOTINIC-ACID FROM 3-CYANOPYRIDINE IN RHODOCOCCUS-RHODOCHROUS J1
NITRILASE-CATALYZED PRODUCTION OF NICOTINIC-ACID FROM 3-CYANOPYRIDINE IN RHODOCOCCUS-RHODOCHROUS J1
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DOI：
10.1128/aem.54.4.1030-1032.1988
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发表时间：
1988-04-01
影响因子：
4.4
通讯作者：
YAMADA, H
中科院分区：
文献类型：
--
作者：
MATHEW, CD;NAGASAWA, T;YAMADA, H
大量存在于Rhodococcus rhodochrous J1细胞中的硝化酶能催化3-氰吡啶直接水解为烟酸，而不形成烟酰胺。利用静息细胞，优化了烟酸生产的反应条件。在最优条件下，加入的3-氰吡啶可100%转化为烟酸，每1.0 ml含有2.89 mg（干重）细胞的反应混合物在26 h内的最高产率为172 mg烟酸。
The nitrilase which occurs abundantly in the cells of Rhodococcus rhodochrous J1 catalyzes the direct hydrolysis of 3-cyanopyridine to nicotinic acid without forming nicotinamide. By using resting cells, the reaction conditions for nicotinic acid production were optimized. Under the optimum conditions, 100% of the added 3-cyanopyridine could be converted to nicotinic acid, the highest yield achieved being 172 mg of nicotinic acid per 1.0 ml of reaction mixture containing 2.89 mg (dry weight) of cells in 26 h.