One-chip sensing device (biomedical photonic LSI) enabled to assess hippocampal steep and gradual up-regulated proteolytic activities
One-chip sensing device (biomedical photonic LSI) enabled to assess hippocampal steep and gradual up-regulated proteolytic activities
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DOI：
10.1016/j.jneumeth.2008.06.002
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发表时间：
2008-08-15
影响因子：
3
通讯作者：
Shiosaka, Sadao
中科院分区：
文献类型：
--
作者：
Tamura, Hideki;Ng, David C.;Shiosaka, Sadao
我们开发了一种植入式单芯片生物荧光成像设备（称为生物医学光子LSI; BpLSI），使实时分子成像与传统的电生理学在体内的脑深部区域。多模式LSI使蛋白水解相关的荧光底物发出的荧光的长期连续成像成为可能。使用BpLSI，我们观察到突触处多位点的刺激依赖性调制过程（16 × 19像素）和高速视频速率下，发现海马CA 1区蛋白水解活性在大范围内逐渐上调，而在局部区域活性陡峭，表明蛋白水解系统是海马中后兴奋突触中长时程增强固定的基础。数学数据分析证实了神经可塑性的功能性蛋白水解的直接参与。(C)2008 Elsevier B. V.保留所有权利。
We developed an implantable one-chip biofluoroimaging device (termed biomedical photonic LSI; BpLSI) which enabled real-time molecular imaging with conventional electrophysiology in vivo in deep brain areas. The multimodal LSI enabled long-term sequential imaging of the fluorescence emitted by proteolysis-linked fluorogenic substrate. Using the BpLSI, we observed a process of stimulation-dependent modulation at synapse with multi-site (16 x 19 pixel) in widespread area and a high-speed video rate, and found that the gradual up-regulated proteolytic activity in a wide range of hippocampal CA1 area and the steep activity in local area, indicating that the proteolysis system is a basis for the fixation of long-term potentiation in post-excited synapses in the hippocampus. Mathematical data analysis confirmed the direct involvement of functional proteolysis for neural plasticity. (C) 2008 Elsevier B.V. All rights reserved.