Survey and analysis of commercial cellulase preparations suitable for biomass conversion to ethanol
Survey and analysis of commercial cellulase preparations suitable for biomass conversion to ethanol
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DOI：
10.1023/a:1008871205580
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发表时间：
1998-03-01
影响因子：
4.1
通讯作者：
Himmel, ME
中科院分区：
文献类型：
--
作者：
Nieves, RA;Ehrman, CI;Himmel, ME
商业纤维素酶已被用于食品、洗涤剂和纺织工业，并且在处理生物质原料方面具有潜在的有效性。研究人员进行了一项调查，以确定美国主要的纤维素酶制造商/经销商，并评估了13种具有代表性的商业制剂的酶活性、蛋白质浓度和化学成分。样品使用滤纸、羧甲基纤维素、纤维素二糖和对硝基苯- β - d -葡萄糖吡喃苷作为底物进行活性测量。为了确定商业制剂的微生物来源，开发了针对里氏木霉CBH I和黑曲霉β - d -葡萄糖苷酶的单克隆抗体的Western blots。其中11个纤维素酶来源于瑞氏或绿氏，2个来源于黑曲霉。
Commercial cellulase enzymes have been used in the food, detergent, and textile industries, and are potentially effective for processing biomass feedstocks. A survey was undertaken to identify major manufacturers/distributors of cellulases in the USA and to evaluate 13 representative commercial preparations for enzyme activity, protein concentration, and chemical composition. Samples were subjected to activity measurements using filter paper, carboxymethylcellulose, cellobiose, and p-nitrophenyl-beta-D-glucopyranoside as substrates. To ascertain the microbial origin of the commercial preparations, Western blots utilizing monoclonal antibodies specific for Trichoderma reesei CBH I and Aspergillus niger beta-D-glucosidase were developed. Eleven of the cellulases tested were of T. reesei or T. viride origin and two were from A. niger.