Atrichodermones A-C, three new secondary metabolites from the solid culture of an endophytic fungal strain, Trichoderma atroviride
Atrichodermones A-C, three new secondary metabolites from the solid culture of an endophytic fungal strain, Trichoderma atroviride
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Atrichodermones A-C，来自内生真菌菌株深绿色木霉固体培养物的三种新的次级代谢产物
DOI：
10.1016/j.fitote.2017.09.012
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发表时间：
2017-11-01
期刊：
影响因子：
3.4
通讯作者：
Zhang, Yonghui
中科院分区：
文献类型：
--
作者：
Zhou, Peng;Wu, Zhaodi;Zhang, Yonghui
从石蒜鳞茎中分离到一株内生真菌，命名为深绿木霉。在水稻培养基上培养后，分离出一种新的3-氨基-5-羟基-5-乙烯基-2-环戊烯-1-酮二聚体，atrichodermone A（1），一种新的环戊烯酮衍生物，atrichodermone B（2），和一种新的倍半萜，atrichodermone C（3），以及三种已知的环戊烯酮衍生物（4-6）。通过大量的光谱（UV、IR、ECD、HRESIMS和NMR）数据分析阐明了它们的结构，并通过将它们的实验ECD光谱与结构相似的化合物和它们的电子圆二色性（ECD）光谱的计算分析比较来确定新化合物的绝对构型。评价化合物1-3对HL 60和U937细胞系的细胞毒性，以及对促炎细胞因子TNF-α和IL-1 β产生的抗炎作用。
An endophytic fungal strain named Trichoderma atroviride was isolated from the bulb of Lycoris radiata. Following cultivation on rice medium, a novel 3-amino-5-hydroxy-5-vinyl-2-cyclopenten-l-one dimer, atrichodermone A (1), a new cyclopentenone derivative, atrichodermone B (2), and a new sesquiterpene, atrichodermone C (3), together with three known cyclopentenone derivatives (4-6) were isolated. Their structures were elucidated by extensive spectroscopic (UV, IR, ECD, HRESIMS, and NMR) data analyses, and absolute configurations of the new compounds were determined by comparing their experimental ECD spectra with structurally similar compounds and computational analyses of their electronic circular dichroism (ECD) spectra. Compounds 1-3 were evaluated for their cytotoxicity against HL60 and U937 cell lines, as well as anti-inflammatory effect against the production of the pro-inflammatory cytokines TNF-alpha and IL-1 beta.