Real-time tracing the changes in the intracellular pH value during apoptosis by near-infrared ratiometric fluorescence imaging
Real-time tracing the changes in the intracellular pH value during apoptosis by near-infrared ratiometric fluorescence imaging
复制标题
近红外比率荧光成像实时追踪细胞凋亡过程中细胞内pH值的变化
DOI：
10.1039/c8cc05385e
复制
发表时间：
2018-08-21
影响因子：
4.9
通讯作者：
Zhao, Shulin
中科院分区：
文献类型：
--
作者：
Lin, Liyun;Zhao, Jingjin;Zhao, Shulin
我们研制了一种单激发双发射的近红外荧光纳米探针。建议的纳米探针应用于实时监测过氧化氢诱导的细胞凋亡过程中的pH值的变化，通过比率成像。H_2O_2（100 μ M）刺激HeLa细胞40分钟后，细胞的pH值由6.6变为5.6。
We developed a near-infrared ratiometric fluorescent nanoprobe with single-excitation dual-emission. The proposed nanoprobe was applied for real-time monitoring the changes in the pH value during hydrogen peroxide-induced apoptosis by ratio imaging. The pH value of HeLa cells changed from 6.6 to 5.6 after H2O2 (100 mu M) stimulation for 40 min.