Anti-obesity effects of glabridin-rich supercritical carbon dioxide extract of licorice in high-fat-fed obese mice
Anti-obesity effects of glabridin-rich supercritical carbon dioxide extract of licorice in high-fat-fed obese mice
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DOI：
10.1016/j.fct.2012.08.048
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发表时间：
2013-01-01
影响因子：
4.3
通讯作者：
Ha, Taeyoul
中科院分区：
文献类型：
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作者：
Ahn, Jiyun;Lee, Hyunjung;Ha, Taeyoul
甘草是一种著名的药用植物，光甘草素是从甘草中分离得到的一种异黄酮类化合物。在本研究中，我们研究了甘草超临界萃取物(LSC)的减肥作用。Glabridin能有效抑制3T3-L1细胞的成脂作用。此外，LSC对脂肪形成的抑制作用呈剂量依赖关系。LSC的抑制作用是通过抑制转录因子CCAAT增强子结合蛋白α和过氧化物酶体增殖物激活受体γ的诱导而实现的。然后用不含LSC、0.1%LSC和0.25%LSC的高脂饲料喂养小鼠8周，探讨LSC的体内减肥作用。LSC以剂量依赖的方式显著减少高脂饮食所致的体重增加。白色脂肪组织肥大程度减轻，脂肪细胞体积缩小。在肝脏中，补充LSC可通过下调糖异生相关的磷酸烯醇式丙酮酸羧激酶和葡萄糖6-磷酸酶，上调β氧化相关的肉碱棕榈酰基转移酶1，从而有效地抑制高脂饮食诱导的肝脏脂肪变性。综上所述，我们的结果表明，光滑素和富含光滑素的甘草提取物将是有效的减肥药物。(C)2012爱思唯尔有限公司。保留所有权利。
Licorice (Glycyrrhiza glabra Linne) is a well-known medicinal plant and glabridin is an isoflavan isolated from licorice. In this study, we investigated the anti-obesity effect of glabridin and glabridin-rich supercritical fluid extract of licorice (LSC). Glabridin effectively inhibited adipogenesis in 3T3-L1 cells. Moreover, LSC showed inhibitory effect on adipogenesis in a dose-dependent manner. The inhibitory effect of LSC resulted from inhibiting the induction of the transcriptional factors CCAAT enhancer binding protein alpha and peroxisome proliferator-activated receptor gamma. Then we fed mice with high-fat diet containing none, 0.1% and 0.25% LSC for 8 weeks to explore the anti-obesity effect of LSC in vivo. LSC significantly reduced weight gain by high-fat diet in a dose-dependent manner. The reductions of the hypertrophy of white adipose tissue and of fat cell size were also observed. In the liver, LSC supplementation effectively inhibited high-fat diet-induced hepatic steatosis through downregulation of gluconeogenesis related phosphoenolpyruvate carboxykinase and glucose 6-phosphatase and upregulation of the beta-oxidation related carnitine palmitoyltransferase 1. Taken together, our results suggest that glabridin and glabridin-rich licorice extract would be effective anti-obesity agents. (c) 2012 Elsevier Ltd. All rights reserved.