Identification and purification of an intrinsic human muscle myogenic factor that enhances muscle repair and regeneration.
Identification and purification of an intrinsic human muscle myogenic factor that enhances muscle repair and regeneration.
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DOI：
10.1006/abbi.2000.2100
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发表时间：
2000-12
影响因子：
3.9
通讯作者：
M. Li;K. Chan;D. Cai;P. Leung;C. Cheng;K. Lee;K. K. Lee-K.
中科院分区：
文献类型：
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作者：
M. Li;K. Chan;D. Cai;P. Leung;C. Cheng;K. Lee;K. K. Lee-K.
严重损伤后受损肌肉再生能力有限是一个主要的临床问题。迄今为止，对于肌肉损伤没有有效的治疗方法。在本研究中，我们研究了粗提物和分级分离的人骨骼肌提取物促进肌原细胞增殖和分化的能力。结果表明，肌肉粗提物能显著刺激C2 C12肌源性细胞系的BrdU参入。此外，该提取物还促进生肌细胞排列和融合。利用电泳技术结合体外复性技术，鉴定出一种分子量约为40 kDa的蛋白质，该蛋白质可产生与肌肉粗提物相同的效果。我们还测试了半纯化（30-50 kDa）肌肉提取物促进成年大鼠肌肉修复的能力。手术干预用于诱导胫骨前肌的肌肉损伤。将半纯化的肌肉提取物（组分H）皮下注射到胫骨前肌上，持续5天。结果发现，受损的肌纤维被新再生的肌纤维所取代。BrdU标记分析显示，这些新再生的纤维来源于分化的卫星细胞的融合。相反，用BSA对照蛋白处理的肌肉的损伤部位主要含有成纤维细胞。
The limited ability of damaged muscle to regenerate after gross injuries is a major clinical problem. To date, there is no effective therapeutic treatment for muscle injuries. In the present study, we have examined the ability of crude and fractionated human skeletal muscle extracts to promote myogenic cell proliferation and differentiation. It was found that the crude muscle extract could significantly stimulate BrdU incorporation in C2C12 myogenic cell line. In addition, the extract also promoted myogenic cell alignment and fusion. Using electrophoresis techniques, in conjunction with in vitro refolding technique, a protein with molecular weight of approximately 40 kDa was identified that could produce the same effects as the crude muscle exdtract. We also tested the ability of semipurified (30-50 kDa) muscle extract to promote muscle repair in adult rats. Surgical intervention was used to induce muscle damage in the tibialis anterior. The semipurified muscle extract (fraction H) was injected subcutaneously over the tibialis anterior for a period of 5 days. It was found that the damaged muscle fibers were replaced by newly regenerated muscle fibers. These newly regenerated fibers originated from the fusion of differentiated satellite cells as revealed by BrdU-labeling analysis. In contrast, the injury site of muscles treated with BSA control protein contained mainly fibroblasts.