Expression of potential target antigens for immunotherapy on primary and metastatic prostate cancers.
Expression of potential target antigens for immunotherapy on primary and metastatic prostate cancers.
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发表时间：
1998-02
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通讯作者：
S. Zhang;H. S. Zhang;V. Reuter;S. Slovin;H. Scher;P. Livingston
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作者：
S. Zhang;H. S. Zhang;V. Reuter;S. Slovin;H. Scher;P. Livingston
确定原发性和转移性前列腺癌上肿瘤相关抗原的表达是选择适当的免疫攻击靶点的关键第一步。在这项研究中，肿瘤相关抗原GM 2、Tn、sTn、T-F、Globo H、Le（y）、MUC 1、MUC 2、MUC 3、MUC 4、MUC 5AC、MUC 5 B、MUC 7、癌胚抗原、人绒毛膜促性腺激素β链的分布采用免疫组化方法比较原发性和转移性前列腺癌及16种正常组织中hCG β、HER 2/neu、PSMA和KSA的表达，使用一组充分表征的单克隆抗体。我们的研究结果表明，GM 2，KSA和MUC 2在9个转移性前列腺癌活检标本中的8个或9个中强烈表达，并且与PSMA，hCG β，TF，Tn和sTn一起，在11个原发性前列腺癌标本中的8个或更多个中强烈表达。Tn、MUC 1和PSMA在9例转移瘤中的4-6例表达。其余的抗原表达不超过三个9转移标本。还用所有抗体测试正常组织。关于在前列腺癌上最广泛表达的八种抗原，PSMA在除前列腺上皮外的任何正常组织上均不显著表达。Tn、sTn、hCG β和MUC 2在10种正常上皮细胞中的3种上被检测到。GM 2、TF、MUC 1和KSA在正常上皮细胞上分布较广，均主要分布在分泌边缘。STn、KSA和hCG β在睾丸中也有表达，GM 2在脑灰质中也有表达。本研究为从筛选出的30种抗原中筛选出GM 2、TF、Tn、sTn、hCG β、MUC 1、MUC 2、KSA和PSMA作为前列腺癌特异性免疫治疗的靶抗原奠定了基础。
Defining the expression of tumor-associated antigens on primary and metastatic prostate cancer is the crucial first step in selecting appropriate targets for immune attack. In this study, the distribution of the tumor-associated antigens GM2, Tn, sTn, Thompson-Friedenreich antigen (TF), Globo H, Le(y), MUC1, MUC2, MUC3, MUC4, MUC5AC, MUC5B, MUC7, carcinoembryonic antigen, beta chain of human chorionic gonadotropin (hCG beta), HER2/neu, PSMA, and KSA on primary and metastatic prostate cancer and 16 types of normal tissues was compared by immunohistochemistry, using a panel of well-characterized monoclonal antibodies. Our results show that GM2, KSA, and MUC2 were strongly expressed on 8 or 9 of 9 metastatic prostate cancer biopsy specimens and, with PSMA, hCG beta, TF, Tn, and sTn, on 8 or more of 11 primary prostate cancer specimens. Tn, MUC1, and PSMA were expressed on 4-6 of 9 metastatic specimens. The remaining antigens were expressed on no more than three of nine metastatic specimens. Normal tissues were also tested with all antibodies. With regard to the eight antigens most widely expressed on prostate cancers, PSMA was not expressed significantly on any of the normal tissues except prostate epithelium. Tn, sTn, hCG beta, and MUC2 were detected on up to 3 of 10 types of normal epithelia. GM2, TF, MUC1, and KSA were more broadly distributed on normal epithelia, all primarily at the secretory borders. STn, KSA, and hCG beta were also detected in the testis, and GM2 was expressed on gray matter of brain. From the 30 antigens that we have screened, this study provides the basis for selecting GM2, TF, Tn, sTn, hCG beta, MUC1, MUC2, KSA, and PSMA as target antigens for specific immunotherapy of prostate cancer.