Separated flows in artificial organs. A cause of early thrombogenesis?
Separated flows in artificial organs. A cause of early thrombogenesis?
复制标题
人造器官中的分离流。
DOI：
10.1097/00002480-199609000-00038
复制
发表时间：
1996
期刊：
影响因子：
--
通讯作者：
Leonard,EF
中科院分区：
文献类型：
--
作者：
Mandrusov,E;Puszkin,E;Vroman,L;Leonard,EF
在人工血液湿化装置中，分离流是不可避免的。被分离的血流结合的表面会导致蛋白质的异常吸附，然后是血小板的黏附和激活，最终导致血栓形成和栓塞。预计会出现长期的异常吸附模式，特别是在分离的流动中，因为在设备启动期间，血液首先会取代润湿液体。作者获得了免疫球蛋白G(Ig G)、纤维蛋白原和高分子激肽原(HMK)在分离流动中和附近的吸附模式。在流动的生理盐水中诱导流动，在时间零时被血浆取代。分离流在4 mm棒后面被引入到稳定的剪切流中(Re=26.4)，在设计的装置中，棒后面的表面是标准的玻璃显微镜载玻片。染色技术显示了每个感兴趣的蛋白质在载玻片表面上的分布，并应用于在引入血浆(最终稀释度为3.5%和8.5%)后停留在流动中的载玻片1、5、10、30和60分钟。结果表明，纤维蛋白原从分离区域附近(而不是在)表面预期迅速消失，并从分隔区域边界的表面延长出现甚至更长时间的消失。从仪器中取出的载玻片，当暴露在血小板悬浮液中时，显示血小板粘连在表面存在纤维蛋白原的地方。
Separated flow is unavoidable in artificial blood-wetted devices. Surfaces bound by separated flows cause abnormal protein adsorption, then platelet adhesion and activation, and eventually thrombogenesis and embolization. A prolonged abnormal adsorption pattern is expected, especially in separated flows, as blood first displaces a wetting liquid during start-up of a device. The authors obtained patterns of immunoglobulin G (IgG), fibrinogen, and high molecular weight kininogen (HMK) adsorption in and near a separated flow. The flow was induced in flowing saline, replaced at time zero by plasma. The separated flow was induced behind a 4 mm bar introduced into a steady shear flow (Re= 26.4) in an apparatus designed so that the surface behind the bar was a standard glass microscope slide. The staining technique revealed the distribution of each protein of interest over the surface of the slide, and was applied to slides residing in the flow for 1, 5, 10, 30, and 60 min after the introduction of plasma (final dilution, 3.5% and 8.5%). Results show the expected, rapid disappearance of fibrinogen from surfaces near (but not in) the separated region, and prolonged appearance and even more prolonged disappearance of fibrinogen from the surface bounding the separated region. Slides removed from the apparatus, when exposed to a platelet suspension, showed that platelets adhered where fibrinogen was present on the surface.