Effect of protein kinase modulators on the induction of morphological differentiation of pheochromocytoma PC12 cells by nerve and fibroblast growth factors
Effect of protein kinase modulators on the induction of morphological differentiation of pheochromocytoma PC12 cells by nerve and fibroblast growth factors
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蛋白激酶调节剂对神经和成纤维细胞生长因子诱导嗜铬细胞瘤PC12细胞形态分化的影响
DOI：
10.1111/j.1470-8744.1990.tb00077.x
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发表时间：
1990
影响因子：
2.8
通讯作者：
A. Kondratyev
中科院分区：
文献类型：
--
作者：
G. Posypanova;SE Severin;A. Kondratyev
研究了蛋白激酶调节剂对神经和成纤维细胞生长因子诱导嗜铬细胞瘤PC12细胞突起生长能力的影响。蛋白激酶抑制剂H7增加了这些因子的神经突起刺激能力。H7的作用在1h内观察到，并呈剂量依赖关系。依赖cAMP和cGMP的蛋白激酶抑制剂HA 1004不影响NGF的轴突刺激活性。抑制蛋白激酶C的物质神经节苷脂GT1b和栎素的作用相似，而鞘氨醇的作用则相反。
The effect of protein kinase modulators on the ability of nerve and fibroblast growth factors to induce neurite outgrowth in pheochromocytoma PC12 cells was studied. The protein kinase inhibitor H7 increased the neurite‐stimulating capacity of these factors. The effect of H7 was observed within 1 h and was dose‐dependent. HA 1004, an inhibitor of cAMP‐ and cGMP‐dependent protein kinases, did not affect the neurite‐stimulating activity of NGF. Substances inhibiting protein kinase C, ganglioside GT1b and quercetin, acted in a similar way whereas sphingosine had an opposite effect.