Low expression of CD39 and CD73 genes in centenarians compared with octogenarians.
Low expression of CD39 and CD73 genes in centenarians compared with octogenarians.
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DOI：
10.1186/s12979-017-0094-3
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发表时间：
2017
期刊：
影响因子：
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通讯作者：
Pintor J
中科院分区：
文献类型：
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作者：
Crooke A;Martínez-Henández J;Martínez-López J;Cruz-Jentoft A;Huete-Toral F;Pintor J
衰老涉及包括免疫系统在内的身体调节系统的逐渐衰退。腺苷通过与其受体相互作用来调节免疫功能，主要是免疫细胞表面的腺苷A2a受体。此外，细胞对这种核苷的反应高度依赖于其胞外浓度，而胞外浓度受胞外酶如CD39和CD73调节。因此，本研究旨在探讨AGE对腺苷A2A受体、CD39和CD73基因表达的影响。用定量聚合酶链式反应检测青年、中老年人和百岁老人外周血中mRNA的变化。与老年人相比，百岁老人CD39、CD73基因表达水平显著降低。关于腺苷A2a受体，我们检测到了高和低转录水平的两个百岁老人亚组。此外，百岁老人的腺苷A2a受体mRNA水平与他们的认知损害无关。总之，我们的初步研究表明，与腺苷A2A受体不同，CD39和CD73的水平可能是人类成功衰老的标志。本文的在线版本(doi：10.1186/s12979-0170094-3)包含补充材料，授权用户可以使用。
Ageing involves a progressive decline of the body’s regulatory systems including immune system. Adenosine regulates immune function by interaction with its receptors, mainly adenosine A2A receptor, present on the surface of immune cells. Furthermore, cellular response to this nucleoside is highly dependent on its extracellular concentration that is regulated by ecto-enzymes such as CD39 and CD73. Therefore, the aim of this study was to investigate the effect of age on adenosine A2A receptor, CD39 and CD73 gene expression. Changes in mRNA were measured by quantitative PCR from peripheral blood of young, middle-aged and older adults as well as centenarians. Centenarians showed a prominent decrease of CD39 and CD73 mRNA in comparison with older adults. Regarding to adenosine A2A receptor, we detected two subgroups of centenarians with high and low level of transcript. Additionally, adenosine A2A receptor mRNA level of centenarians, did not correlate with their cognitive impairment. In summary, our pilot study suggests that unlike of adenosine A2A receptor, the level of CD39 as well as CD73 mRNA could be a hallmark of successful human ageing. The online version of this article (doi:10.1186/s12979-017-0094-3) contains supplementary material, which is available to authorized users.