2'-hydroxypyridoxol, a biosynthetic precursor of vitamins B(6) and B(1) in yeast.
2'-hydroxypyridoxol, a biosynthetic precursor of vitamins B(6) and B(1) in yeast.
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2-羟基吡哆醇，酵母中维生素 B(6) 和 B(1) 的生物合成前体。
DOI：
10.1021/ja012708z
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发表时间：
2002
影响因子：
15
通讯作者：
Spenser,IanD
中科院分区：
文献类型：
--
作者：
Zeidler,Johannes;Ullah,Nisar;Gupta,RamNath;Pauloski,RichardM;Sayer,BrianG;Spenser,IanD
In separate experiments cultures of the yeastSaccharomyces cerevisiaeATTC 7752 were grown in the presence of [5‘,5‘-2H2]- or of [2‘,2‘,5‘,5‘-2H4]-3-hydroxy-2,4,5-tri(hydroxymethyl)pyridine (i.e., 2‘-hydroxypyridoxol). The2H NMR spectra of the samples of pyridoxamine dihydrochloride and of thiamin chloride hydrochloride that were isolated from the two experiments showed the presence of deuterium at the corresponding sites. Entry of deuterium from the specifically2H-labeled samples of 2‘-hydroxypyridoxol into the predicted sites of pyridoxamine and of the pyrimidine unit of thiamin provides the first unequivocal evidence that, in yeast, 2‘-hydroxypyridoxol is an intermediate on the route from a C5-sugar into vitamin B6, and adds to the evidence that pyridoxol serves as a precursor of the pyrimidine unit of thiamin, supplying the C5N unit, C-2‘,2,N-1,C-6,5,5‘ as an intact unit.
