Effects of GABA receptor blockage on the respiratory response to hypoxia in sedated newborn piglets.
Effects of GABA receptor blockage on the respiratory response to hypoxia in sedated newborn piglets.
复制标题
GABA 受体阻断对镇静新生仔猪缺氧呼吸反应的影响。
DOI：
10.1152/jappl.1994.77.2.1006
复制
发表时间：
1994
影响因子：
3.3
通讯作者：
Eduardo Bancalari
中科院分区：
文献类型：
--
作者：
Jian Huang;C. Suguihara;D. Hehre;Jing Lin;Eduardo Bancalari
脑γ -氨基丁酸（GABA）水平在缺氧时增加，这可能调节对缺氧的通气反应。为了验证新生儿缺氧通气反应下降是否与中枢神经系统GABA活性升高有关，研究了26只镇静自主呼吸的新生仔猪（5 +/- 1日龄，体重1.7 +/- 0.4 kg）。在室内空气中以及药物干预前缺氧1、5、10 min（吸入氧分数0.10）后，测量分钟通气量（VE）、耗氧量、心率、动脉血压和动脉血气。在这些测量之后，立即在动物缺氧时输注生理盐水或GABA α受体阻滞剂（双库兰，0.3 mg/kg iv）或β受体阻滞剂（CGP-35348, 100-300 mg/kg iv）。在开始药物输注后1、5和10分钟重复所有测量。各组间基本VE相似。在缺氧期间，接受GABA α受体阻滞剂或β受体阻滞剂的动物的VE显著增加，而接受生理盐水的动物则没有。生理盐水或GABA拮抗剂输注前后各组动脉Po2、耗氧量、心率和动脉血压的变化相似。这些结果表明，新生儿仔猪缺氧双相通气反应中通气量的减少部分是通过GABA对中枢神经系统的抑制作用介导的。
Brain gamma-aminobutyric acid (GABA) levels increase during hypoxia, which may modulate the ventilatory response to hypoxia. To test the possibility that the depressed neonatal ventilatory response to hypoxia may be related to increased central nervous system GABA activity, 26 sedated spontaneously breathing newborn piglets (age 5 +/- 1 day, wt 1.7 +/- 0.4 kg) were studied. Minute ventilation (VE), oxygen consumption, heart rate, arterial blood pressure, and arterial blood gases were measured in room air and after 1, 5, and 10 min of hypoxia (inspired O2 fraction 0.10) before drug intervention. Immediately after these measurements, an infusion of saline or the GABA alpha-receptor blocker (bicuculline, 0.3 mg/kg iv) or beta-receptor blocker (CGP-35348, 100-300 mg/kg iv) was administered while animals were hypoxic. All measurements were repeated at 1, 5, and 10 min after initiation of the drug infusion. Basal VE was similar among groups. During hypoxia, VE increased significantly in the animals that received either a GABA alpha- or beta-receptor blocker but not in those receiving saline. Changes in arterial Po2, oxygen consumption, heart rate, and arterial blood pressure were similar among groups before and after saline or GABA antagonist infusion. These results suggest that the decrease in ventilation during the biphasic ventilatory response to hypoxia in the neonatal piglet is in part mediated through the depressant effect of GABA on the central nervous system.