Modulation of Phase II Enzymes by Sulforaphane: Implications for Its Cardioprotective Potential
Modulation of Phase II Enzymes by Sulforaphane: Implications for Its Cardioprotective Potential
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DOI：
10.1021/jf900549c
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发表时间：
2009-06-24
影响因子：
6.1
通讯作者：
Hrelia, Silvana
中科院分区：
文献类型：
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作者：
Angeloni, Cristina;Leoncini, Emanuela;Hrelia, Silvana
氧化应激在心脏疾病的病理生理学中起着重要作用，但外源性抗氧化剂的保护作用的实验数据是有争议的。一个有前途的心脏保护策略可能是通过诱导内源性抗氧化剂和II相酶的化学诱导剂。萝卜硫烷是一种异硫氰酸酯，来源于十字花科蔬菜，它已获得关注，主要是作为一个潜在的化学预防剂，部分通过诱导解毒酶。因此，本研究旨在研究莱菔硫烷对培养的新生大鼠心肌细胞中抗氧化剂和II相酶[谷胱甘肽还原酶、谷胱甘肽-S-转移酶、谷胱甘肽过氧化物酶、NAD（P）H：醌氧化还原酶-1、硫氧还蛋白还原酶]的基因转录、蛋白表达和酶活性的时间依赖性诱导。萝卜硫素的潜在心脏保护作用通过细胞内活性氧产生的减少、细胞活力的增加以及长期治疗后伴随着心肌细胞中抗氧化剂和II相酶的诱导的DNA片段化的减少来证实。
Oxidative stress plays a major role in the pathophysiology of cardiac disorders, but the experimental data on the protective effects, of exogenous antioxidants are controversial. A promising cardioprotective strategy may be through the induction of the endogenous antioxidants and phase II enzymes by chemical inducers. Sulforaphane is an isothiocyanate derived from cruciferous vegetables, and it has gained attention mainly as a potential chemopreventive agent in part through the induction of detoxifying enzymes. Accordingly, this study was undertaken to investigate the time-dependent induction of gene transcription, protein expression, and enzyme activity of antioxidant and phase II enzymes [glutathione reductase, glutathione-S-transferase, glutathione peroxidase, NAD(P)H:quinone oxidoreductase-1, thioredoxin reductase] by sulforaphane in cultured rat neonatal cardiomyocytes. The potential cardioprotective action of sulforaphane was confirmed by the decrease in intracellular reactive oxygen species production, the increase in cell viability, and the decrease in DNA fragmentation after long-term treatment accompanied by the induction of antioxidants and phase II enzymes in cardiomyocytes.