The role of carbohydrates in the induction of flowering in Arabidopsis thaliana:: comparison between the wild type and a starchless mutant
The role of carbohydrates in the induction of flowering in Arabidopsis thaliana:: comparison between the wild type and a starchless mutant
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DOI：
10.1007/s004250050383
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发表时间：
1998-09-01
期刊：
影响因子：
4.3
通讯作者：
Bernier, G
中科院分区：
文献类型：
--
作者：
Corbesier, L;Lejeune, P;Bernier, G
为了验证叶片碳水化合物输出增加和淀粉动员是否是拟南芥花转变的关键，对哥伦比亚生态型及其无淀粉突变体pgm和淀粉过量突变体sex1进行了研究。通过将植物暴露于一个长日（LD）或一个移位短日（DSD）来实现开花的诱导。结果表明：（1）pgm和sex1突变体在短于16 h的光照条件下均表现为晚花表型。(ii)当诱导处理引起大百分比的诱导植物时，总是存在从叶片输出的碳水化合物的大的、早期的和瞬时的增加。相比之下，当诱导处理仅导致低百分比的诱导植物（暴露于一种DSD的pgm植物）时，来自叶的碳水化合物的输出不增加，支持韧皮部碳水化合物在花转变中具有关键功能的观点。(iii)当诱导是通过一个LD（延长的光合作用期）时，淀粉动员不需要获得增加的碳水化合物输出，但当诱导是通过一个DSD（不受影响的光合作用期）时，淀粉动员是绝对必要的。(iv)成花诱导明显提高了叶片韧皮部装载系统的能力。
In order to test whether an increased export of carbohydrates by leaves and starch mobilization are critical for floral transition in Arabidopsis thaliana, the Columbia ecotype as well as its starchless mutant pgm and starch-in-excess mutant sex1 were investigated. Induction of flowering was achieved by exposure of plants to either one long day (LD) or one displaced short day (DSD). The following conclusions were drawn: (i) Both the pgm and sex1 mutants have a late-flowering phenotype in days shorter than 16 h. (ii) When inductive treatments cause a large percentage of induced plants, there is always a large, early and transient increase in carbohydrate export from leaves. By contrast, when an inductive treatment results in only a low percentage of induced plants (pgm plants exposed to one DSD), the export of carbohydrates from leaves is not increased, supporting the idea that phloem carbohydrates have a critical function in floral transition. (iii) Starch mobilization is not required to obtain an increased carbohydrate export when induction is by one LD (extended period of photosynthesis), but is absolutely essential when induction is by one DSD (period of photosynthesis unaffected). (iv) Floral induction apparently increases the capability of the leaf phloem-loading system.