Heterologous Overexpression of Poplar SnRK2 Genes Enhanced Salt Stress Tolerance in Arabidopsis thaliana.
Heterologous Overexpression of Poplar SnRK2 Genes Enhanced Salt Stress Tolerance in Arabidopsis thaliana.
复制标题
杨树SnRK2基因异源过度表达增强拟南芥盐胁迫耐受性
DOI：
10.3389/fpls.2016.00612
复制
发表时间：
2016
影响因子：
5.6
通讯作者：
Zhuge Q
中科院分区：
文献类型：
--
作者：
Song X;Yu X;Hori C;Demura T;Ohtani M;Zhuge Q
SNF 1相关蛋白激酶亚家族2（SnRK 2）作为脱落酸信号传导的全局性正调控因子，在植物非生物胁迫应答中发挥重要作用。在模式树大果杨的基因组中，已经鉴定了12个SnRK 2基因，并且一些基因被非生物胁迫上调。在这项研究中，我们异源过表达的PtSnRK 2基因在拟南芥中，发现PtSnRK2.5和PtSnRK2.7基因的过表达增强胁迫耐受性。在PtSnRK2.5和PtSnRK2.7过表达，叶绿素含量，根伸长保持在盐胁迫条件下，导致较高的存活率在盐胁迫下与野生型相比。转录组学分析表明，PtSnRK2.7过表达影响应激相关代谢基因，包括脂质代谢和类黄酮代谢，即使在正常生长条件下。然而，在拟南芥SRK 2C/SnRK2.6和小麦SnRK2.8过表达中被报道上调的应激反应基因并没有被PtSnRK2.7过表达改变。此外，PtSnRK2.7过表达广泛，并在很大程度上影响了响应盐胁迫的转录组;与运输活性相关的基因，包括阴离子转运相关基因，特征性地上调，多种代谢基因特异性下调。我们还发现，盐胁迫反应基因在PtSnRK2.7过表达中大大上调。两者合计，白杨亚类2 PtSnRK 2基因可以调节拟南芥盐胁迫耐受性，通过激活细胞信号转导途径以不同的方式从草本植物亚类2 SnRK 2基因。
Subfamily 2 of SNF1-related protein kinase (SnRK2) plays important roles in plant abiotic stress responses as a global positive regulator of abscisic acid signaling. In the genome of the model tree Populus trichocarpa, 12 SnRK2 genes have been identified, and some are upregulated by abiotic stresses. In this study, we heterologously overexpressed the PtSnRK2 genes in Arabidopsis thaliana and found that overexpression of PtSnRK2.5 and PtSnRK2.7 genes enhanced stress tolerance. In the PtSnRK2.5 and PtSnRK2.7 overexpressors, chlorophyll content, and root elongation were maintained under salt stress conditions, leading to higher survival rates under salt stress compared with those in the wild type. Transcriptomic analysis revealed that PtSnRK2.7 overexpression affected stress-related metabolic genes, including lipid metabolism and flavonoid metabolism, even under normal growth conditions. However, the stress response genes reported to be upregulated in Arabidopsis SRK2C/SnRK2.6 and wheat SnRK2.8 overexpressors were not changed by PtSnRK2.7 overexpression. Furthermore, PtSnRK2.7 overexpression widely and largely influenced the transcriptome in response to salt stress; genes related to transport activity, including anion transport-related genes, were characteristically upregulated, and a variety of metabolic genes were specifically downregulated. We also found that the salt stress response genes were greatly upregulated in the PtSnRK2.7 overexpressor. Taken together, poplar subclass 2 PtSnRK2 genes can modulate salt stress tolerance in Arabidopsis, through the activation of cellular signaling pathways in a different manner from that by herbal subclass 2 SnRK2 genes.